Anti-DNA Polymerase epsilon抗体(ab83442)

概述

  • 产品名称Anti-DNA Polymerase epsilon抗体
    参阅全部 DNA Polymerase epsilon 一抗
  • 描述
    兔多克隆抗体to DNA Polymerase epsilon
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 (AERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATS C) of Human POLE3 (NP_059139).

  • 阳性对照
    • Human fetal lung lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.Can be blocked with DNA Polymerase epsilon peptide (ab135296). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based assay is 1/1562500.

靶标

  • 功能Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
  • 组织特异性Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Arsenic transactivated protein antibody
    • Arsenic-transactivated protein antibody
    • ASTP antibody
    • CHARAC 17 antibody
    • CHARAC17 antibody
    • CHRAC 17 antibody
    • CHRAC-17 antibody
    • CHRAC17 antibody
    • Chromatin Accessibility Complex 17 antibody
    • Chromatin accessibility complex 17 kDa protein antibody
    • DNA Polymerase Epsilon p17 Subunit antibody
    • DNA polymerase epsilon subunit 3 antibody
    • DNA polymerase epsilon subunit p17 antibody
    • DNA polymerase II subunit 3 antibody
    • DPOE3_HUMAN antibody
    • Histone fold protein CHRAC17 antibody
    • HuCHRAC 17 antibody
    • HuCHRAC17 antibody
    • p17 antibody
    • p17 subunit antibody
    • POL E3 antibody
    • POLE 3 antibody
    • POLE3 antibody
    • Polymerase (DNA directed) epsilon 3 antibody
    • YBL 1 antibody
    • YBL1 antibody
    see all

Anti-DNA Polymerase epsilon antibody 图像

  • Anti-DNA Polymerase epsilon antibody (ab83442) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody.

    Predicted band size : 16 kDa

Anti-DNA Polymerase epsilon antibody (ab83442)参考文献

ab83442 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.
We have now been able to add the peptide used to generate ab83442 to the catalogue.
It has been added as: DNA Polymerase epsilon peptide (ab135296)
If there is anything else I can help you with, please let m...

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