Anti-CHRAC-17抗体(ab74308)
Key features and details
- Rabbit polyclonal to CHRAC-17
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CHRAC-17抗体
参阅全部 CHRAC-17 一抗 -
描述
兔多克隆抗体to CHRAC-17 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat -
免疫原
Synthetic peptide within Human CHRAC-17 aa 97-147. The exact sequence is proprietary. NP_059139.2
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阳性对照
- WB: Lysate from HeLa or HEK-293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74308于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 16 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 16 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. -
组织特异性
Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 510678 Cow
- Entrez Gene: 54107 Human
- Entrez Gene: 59001 Mouse
- Entrez Gene: 100362333 Rat
- Entrez Gene: 298098 Rat
- Omim: 607267 Human
- SwissProt: Q3SZN5 Cow
- SwissProt: Q9NRF9 Human
see all -
别名
- Arsenic transactivated protein antibody
- Arsenic-transactivated protein antibody
- ASTP antibody
see all
图片
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All lanes : Anti-CHRAC-17 antibody (ab74308) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from HEK-293T cells at 50 µg
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labeling CHRAC-17 with ab74308 at 1/5000 (0.2 µg/ml). Detection: DAB.
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Detection of human CHRAC-17 by immunoprecipitation of 1mg HeLa cells whole lysate, using ab74308 at 3µg/mg lysate (lane 1, 20% IP loaded) or a control IgG (lane 2, 20% IP loaded). The post-IP WB was performed using ab74308 at 1 µg/ml.
Detection: chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab74308 被引用在 1 文献中.
- Wu R et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB . PubMed: 27679476