ab38338 gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
A431 (Human epithelial carcinoma cell line),
HEK 293 (Human embryonic kidney cell line),
HepG2 (Human hepatocellular liver carcinoma cell line),
MCF-7 (Human breast adenocarcinoma cell line).
and the following Nuclear lysate:
HeLa (Human epithelial carcinoma cell line).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Predicted band size : 51 kDa Observed band size : 51 kDa Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - DNA polymerase delta p50 antibody (ab38338)
ICC/IF image of ab38338 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38338, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Karkhanis V et al. Protein Arginine Methyltransferase 7 Regulates Cellular Response to DNA Damage by Methylating Promoter Histones H2A and H4 of the Polymerase d Catalytic Subunit Gene, POLD1. J Biol Chem287:29801-14 (2012).
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