ab38338 gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
A431 (Human epithelial carcinoma cell line),
HEK 293 (Human embryonic kidney cell line),
HepG2 (Human hepatocellular liver carcinoma cell line),
MCF-7 (Human breast adenocarcinoma cell line).
and the following Nuclear lysate:
HeLa (Human epithelial carcinoma cell line).
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Western blot - DNA polymerase delta p50 antibody (ab38338)
All lanes : Anti-DNA polymerase delta p50 antibody (ab38338) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 :Jurkat whole cell lysate (ab7899) Lane 4 :A431 whole cell lysate (ab7909) Lane 5 :HEK293 whole cell lysate (ab7902) Lane 6 :HepG2 whole cell lysate (ab7900) Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa Observed band size: 51 kDa Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - DNA polymerase delta p50 antibody (ab38338)
ICC/IF image of ab38338 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38338, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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