Anti-DNA Polymerase delta, catalytic subunit抗体[607] (ab10362)

概述

  • 产品名称Anti-DNA Polymerase delta, catalytic subunit抗体[607]
    参阅全部 DNA Polymerase delta, catalytic subunit 一抗
  • 描述
    小鼠单克隆抗体[607] to DNA Polymerase delta, catalytic subunit
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant protein, corresponding to amino acids 1-140 of the large subunit of Human DNA Polymerase delta.

  • 阳性对照
    • HeLa whole cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Antibody purified from hybridoma cell culture supernatant by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.
  • 克隆单克隆
  • 克隆编号607
  • 骨髓瘤NS1
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
IP Use a concentration of 5 µg/ml.

靶标

  • 功能Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
  • 序列相似性Belongs to the DNA polymerase type-B family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Polymerase (DNA directed) delta 1 catalytic subunit antibody
    • CDC2 antibody
    • CDC2 homolog antibody
    • CRCS10 antibody
    • DNA directed DNA polymerase delta 1 antibody
    • DNA directed polymerase delta 1 antibody
    • DNA pol delta 1 antibody
    • DNA polymerase delta catalytic subunit antibody
    • DNA polymerase subunit delta p125 antibody
    • DPOD1_HUMAN antibody
    • MDPL antibody
    • POLD antibody
    • POLD 1 antibody
    • POLD1 antibody
    • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa antibody
    • Polymerase (DNA) delta 1 catalytic subunit antibody
    • Polymerase DNA directed delta 1 catalytic subunit 125kD antibody
    • polymerase, DNA, delta antibody
    see all

Anti-DNA Polymerase delta, catalytic subunit antibody [607] 图像



  • Predicted band size : 130 kDa


    Detection of human DNA polymerase delta by using anti-pol delta monoclonal antibody [607] (ab10362) in HeLa whole cell extract.
  • DNA Polymerase delta, catalytic subunit was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to DNA Polymerase delta, catalytic subunit and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10362.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 130kDa: DNA Polymerase delta, catalytic subunit

Anti-DNA Polymerase delta, catalytic subunit antibody [607] (ab10362)参考文献

This product has been referenced in:
  • Anderson DD  et al. Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J Biol Chem 287:7051-62 (2012). IP ; Human . Read more (PubMed: 22235121) »
  • Kawabata T  et al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome 22:506-17 (2011). WB ; Mouse . Read more (PubMed: 21611832) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (1 TC28, 2 HeLa)
Loading amount 15 µg
Specification 1 TC28, 2 HeLa
Gel Running Conditions Reduced Denaturing (8%)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 16 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa whole cell lysate)
Loading amount 150 µg
Specification HeLa whole cell lysate
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Michael Mcilwraith

Verified customer

提交于 Apr 26 2006

Thank you for your enquiry. For ab10362, I have added an image of the antibody used in Western blot to the datasheet. For ab104, I have added a link to a reference using the same clone in Western blot and Northern blot. Please follow the links bel...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"