The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: Ready to use for 60 min at room temperature .
Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
序列相似性Belongs to the DNA polymerase type-X family.
结构域Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
翻译后修饰Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.