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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNA Polymerase beta aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P06746
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab175197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 38 kDa.|
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Immunofluorescence analysis of A431 cells labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling DNA Polymerase beta with ab175197 at 1/10 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"