重组
RabMAb

Anti-DNA Polymerase beta抗体[EPR12722(B)] (ab175197)

概述

  • 产品名称
    Anti-DNA Polymerase beta抗体[EPR12722(B)]
    参阅全部 DNA Polymerase beta 一抗
  • 描述
    兔单克隆抗体[EPR12722(B)] to DNA Polymerase beta
  • 经测试应用
    适用于: ICC/IF, IP, IHC-P, WBmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human DNA Polymerase beta aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P06746

  • 阳性对照
    • HeLa, Jurkat, K562 and A431 cell lysates; Human testis and uterus tissues; A431 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab175197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
    • 序列相似性
      Belongs to the DNA polymerase type-X family.
    • 结构域
      Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
    • 翻译后修饰
      Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
    • 细胞定位
      Nucleus, Cytoplasm
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA directed DNA polymerase beta antibody
      • DNA pol beta antibody
      • DNA polymerase beta antibody
      • DNA polymerase beta subunit antibody
      • DPOLB_HUMAN antibody
      • MGC125976 antibody
      • Pol B antibody
      • Pol beta antibody
      • POLB antibody
      • Polymerase (DNA directed) beta antibody
      see all

    图片

    • All lanes : Anti-DNA Polymerase beta antibody [EPR12722(B)] (ab175197) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : Jurkat cell lysates
      Lane 3 : K562 cell lysates
      Lane 4 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 38 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

    • Immunofluorescence analysis of A431 cells labeling DNA Polymerase beta with ab175197 at a 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling DNA Polymerase beta with ab175197 at 1/10 dilution.

    文献

    This product has been referenced in:
    • Lai Y  et al. Crosstalk between MSH2-MSH3 and polß promotes trinucleotide repeat expansion during base excision repair. Nat Commun 7:12465 (2016). Read more (PubMed: 27546332) »
    • Wang H  et al. Cell cycle regulation of DNA polymerase beta in rotenone-based Parkinson's disease models. PLoS One 9:e109697 (2014). Human . Read more (PubMed: 25303312) »

    See all 2 Publications for this product

    客户评价及客户问答

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (spermatocytes)
    Permeabilization
    No
    Specification
    spermatocytes
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Prasada Rao

    Verified customer

    提交于 Jun 27 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Spermatocytes)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    30 µg
    Specification
    Spermatocytes
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 27 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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