概述

  • 产品名称Anti-DNA Polymerase beta抗体
    参阅全部 DNA Polymerase beta 一抗
  • 描述
    兔多克隆抗体to DNA Polymerase beta
  • 特异性This antibody detects endogenous levels of total DNA polymerase beta.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized peptide derived from human DNA Polymerase beta.

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue. WB: Extracts from NIH/3T3 cells.

性能

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应用

Our Abpromise guarantee covers the use of ab53059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • 序列相似性Belongs to the DNA polymerase type-X family.
  • 结构域Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • 翻译后修饰Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA directed DNA polymerase beta antibody
    • DNA pol beta antibody
    • DNA polymerase beta antibody
    • DNA polymerase beta subunit antibody
    • DPOLB_HUMAN antibody
    • MGC125976 antibody
    • Pol B antibody
    • Pol beta antibody
    • POLB antibody
    • Polymerase (DNA directed) beta antibody
    see all

Anti-DNA Polymerase beta antibody 图像

  • All lanes : Anti-DNA Polymerase beta antibody (ab53059) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells
    Lane 2 : Extracts from NIH/3T3 cells treated with peptide


    Predicted band size : 37 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of DNA Polymerase beta in paraffin embedded human breast carcinoma tissue using ab53059 at a 1/50 dilution.
    Left image: untreated.
    Right image: treated with peptide.

Anti-DNA Polymerase beta antibody (ab53059)参考文献

This product has been referenced in:
  • Yoshizawa K  et al. Gastrointestinal hyperplasia with altered expression of DNA polymerase beta. PLoS One 4:e6493 (2009). IHC-P ; Human . Read more (PubMed: 19654874) »

See 1 Publication for this product

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