Anti-DNA Polymerase beta抗体(ab26343)

概述

  • 产品名称
    Anti-DNA Polymerase beta抗体
    参阅全部 DNA Polymerase beta 一抗
  • 描述
    兔多克隆抗体to DNA Polymerase beta
  • 经测试应用
    适用于: IP, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human DNA Polymerase beta aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.

  • 阳性对照
    • This antibody gave a positive control in the following human whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) This antibody gave a positive control in the following mouse embryonic fibroblast cell line lysates: NIH 3T3 whole cell MEF1 whole cell This antibody gave a positive control in the following rat whole cell lysate: PC12 (Rat adrenal pheochromocytoma cell line)

性能

应用

Our Abpromise guarantee covers the use of ab26343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

图片

  • All lanes : Anti-DNA Polymerase beta antibody (ab26343) at 0.5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 150 kDa (possible cross reactivity),48 kDa (possible cross reactivity),90 kDa (possible cross reactivity).
  • Formaldehyde-fixed human small cell lung cancer tissue stained for DNA Polymerase beta using ab26343 at 1/200 dilution in immunohistochemical analysis.

    See Abreview

  • All lanes : Anti-DNA Polymerase beta antibody (ab26343) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 120 kDa (possible cross reactivity),30 kDa (possible cross reactivity).
  • Image courtesy of Human Protein Atlas ab26343 staining DNA polymerase beta in Human gall bladder, showing a strong nuclear staining pattern in the columnar epithelial cells in contrast with the cells in the lamina propria. Paraffin embedded human gall bladder tissue was incubated with ab26343 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab26343 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab26343 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26343 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Sun H  et al. The FEN1 L209P mutation interferes with long-patch base excision repair and induces cellular transformation. Oncogene 36:194-207 (2017). Read more (PubMed: 27270424) »
  • Kirby TW  et al. DNA polymerase ß contains a functional nuclear localization signal at its N-terminus. Nucleic Acids Res 45:1958-1970 (2017). ICC/IF . Read more (PubMed: 27956495) »

See all 15 Publications for this product

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (Human Embryonic Kidney cells 293)
Negative control
IgG control
Specification
Human Embryonic Kidney cells 293
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 30% Formaldehyde
Positive control
Input control
Username

Abcam user community

Verified customer

提交于 Nov 18 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
20 µg
Specification
HeLa
Treatment
siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 May 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Embryonic stem cell)
Loading amount
100 µg
Specification
Embryonic stem cell
Gel Running Conditions
Reduced Denaturing (8% page gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Small Cell Lung Cancer)
Total protein in input
2e+006 cells
Specification
Small Cell Lung Cancer
Immuno-precipitation step
Other - Protein G Dynabeads
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Abcam user community

Verified customer

提交于 Nov 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Small Cell Lung Cancer)
Specification
Small Cell Lung Cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Bond ER1
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Nov 18 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Small Cell Lung Cancer)
Loading amount
15 µg
Specification
Small Cell Lung Cancer
Treatment
POLB overexpressed
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
Loading amount
15 µg
Specification
1 EBNA, 2 HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%
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Abcam user community

Verified customer

提交于 Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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