Anti-DNA Polymerase beta抗体[61] (ab1831)


  • 产品名称Anti-DNA Polymerase beta抗体[61]
    参阅全部 DNA Polymerase beta 一抗
  • 描述
    小鼠单克隆抗体[61] to DNA Polymerase beta
  • 经测试应用适用于: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    BALB/C mice were injected with DNA polymerase beta protein.

  • 阳性对照
    • Testis



Our Abpromise guarantee covers the use of ab1831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. (PMID 20019666).
Flow Cyt Use 1µg for 106 cells.


  • 功能Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • 序列相似性Belongs to the DNA polymerase type-X family.
  • 结构域Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • 翻译后修饰Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • 细胞定位Nucleus, Cytoplasm
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA directed DNA polymerase beta antibody
    • DNA pol beta antibody
    • DNA polymerase beta antibody
    • DNA polymerase beta subunit antibody
    • DPOLB_HUMAN antibody
    • MGC125976 antibody
    • Pol B antibody
    • Pol beta antibody
    • POLB antibody
    • Polymerase (DNA directed) beta antibody
    see all

Anti-DNA Polymerase beta antibody [61] 图像

  • ab1831 staining human testis by IHC-P.
  • Overlay histogram showing Jurkat cells stained with ab1831 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1831, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • All lanes : Anti-DNA Polymerase beta antibody [61] (ab1831)

    Lane 1 : Empty vector
    Lane 2 : Wild-type mouse embryonic fibroblasts
    Lane 3 : G231D mutant mouse embryonic fibroblasts
    Lane 4 : T292I mutant mouse embryonic fibroblasts
    Lane 5 : S275N/E295K mutant mouse embryonic fibroblasts

    Predicted band size : 38 kDa

    Image from Donigan KA et al., J Biol Chem. 2012 Jul 6;287(28):23830-9. Epub 2012 May 10.; Fig S1.; doi: 10.1074/jbc.M111.324947; July 6, 2012, The Journal of Biological Chemistry, 287, 23830-23839.

Anti-DNA Polymerase beta antibody [61] (ab1831)参考文献

This product has been referenced in:
  • Xu B  et al. The changes of 8-OHdG, hOGG1, APE1 and Pol ß in lenses of patients with age-related cataract. Curr Eye Res 40:378-85 (2015). WB . Read more (PubMed: 24911554) »
  • Stilling RM  et al. K-Lysine acetyltransferase 2a regulates a hippocampal gene expression network linked to memory formation. EMBO J 33:1912-27 (2014). Mouse . Read more (PubMed: 25024434) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Small Cell Lung Cancer)
Loading amount 5 µg
Specification Small Cell Lung Cancer
Treatment POLB over expressed
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Oct 15 2009

Thank you for your email. The antibody solution consists of 1X PBS, pH 7.5; 1% BSA; and 0.5 % sodium azide. Please contact us again if you have any additional questions.

The storage buffer is PBS, stabilizer is BSA and preservative is Sodium azide. Please contact us again if you have any additional questions.

Thank you for contacting us for technical support with ab1831. This antibody has not been tested on mouse samples and we would not predict it to work on mouse tissue samples in immunohistochemical techniques due to the problem of mouse-on-mouse stai...

Read More

Thank you for your enquiry. This antibody was purified by protein A chromatography, and the concentration is approximately 100 ug/ml. If you have any additional questions, please contact us again.

I'm sorry to hear you are having problems with ab1831 in IHC. The fact that the antibody still works in WB suggests that the antibody is fine but that there is a component in the IHC protocol which is damaged, there could potentially be many "ingre...

Read More

Thank you for the information. We have not received any other complaints regarding this antibody and since the previous vial of ab1831 worked for you, it sounds like perhaps there is a problem with the latest vial that you received. I'm sending you a r...

Read More

Thank you for your email. I can send you a replacement vial of ab1831, it is no problem. But can you be more specific regarding the samples that you are using? Also, when approximately did you order the vial of ab1831 that did work for you? That inform...

Read More