Anti-DNA polymerase alpha抗体(ab65009)


  • 产品名称Anti-DNA polymerase alpha抗体
    参阅全部 DNA polymerase alpha 一抗
  • 描述
    兔多克隆抗体to DNA polymerase alpha
  • 特异性This antibody detects endogenous levels of total DNA polymerase alpha protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide derived from N-terminal of human DNA polymerase alpha

  • 阳性对照
    • Extracts from Jurkat, 293 and HepG2 cells


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab65009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 169 kDa (predicted molecular weight: 169 kDa).
ELISA 1/20000.


  • 功能Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
  • 序列相似性Belongs to the DNA polymerase type-B family.
  • 翻译后修饰A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686K1672 antibody
    • DNA polymerase alpha 1 catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit p180 antibody
    • DNA polymerase alpha p180 subunit antibody
    • DPOLA_HUMAN antibody
    • OTTHUMP00000023089 antibody
    • OTTHUMP00000023090 antibody
    • P180 antibody
    • p180 subunit antibody
    • POLA antibody
    • POLA1 antibody
    • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
    • Polymerase (DNA-directed), alpha (70kD) antibody
    see all

Anti-DNA polymerase alpha antibody 图像

  • All lanes : Anti-DNA polymerase alpha antibody (ab65009) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from HepG2 cells
    Lane 4 : Extracts from HepG2 cells with immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 169 kDa
    Observed band size : 169 kDa

Anti-DNA polymerase alpha antibody (ab65009)参考文献

ab65009 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.
For ab57296, the sequence homology of the immunogen with mouse is 83% - which is good.
The lab let me know that for ab65009, the amino acid range of the immunogen is within residues 100-300 of human DNA Polymerase ...

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I am checking with the lab about the homology of the immunogen sequence with mouse. The overall homology between human and mouse is 88%, but some regions align better than others.
I will let you know when I hear back.
What applications are...

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