Anti-DNA polymerase alpha抗体(ab57296)

概述

  • 产品名称Anti-DNA polymerase alpha抗体
    参阅全部 DNA polymerase alpha 一抗
  • 描述
    小鼠单克隆抗体to DNA polymerase alpha
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: QFSRTGPLCP ACMKATLQPE YSDKSLYTQL CFYRYIFDAE CALEKLTTDH EKDKLKKQFF TPKVLQDYRK LKNTAEQFLS RSGYSEVNLS KLFAGCAVKS , corresponding to amino acids 1363-1463 of Human DNA polymerase alpha

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
    • 序列相似性Belongs to the DNA polymerase type-B family.
    • 翻译后修饰A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686K1672 antibody
      • DNA polymerase alpha 1 catalytic subunit antibody
      • DNA polymerase alpha catalytic subunit antibody
      • DNA polymerase alpha catalytic subunit p180 antibody
      • DNA polymerase alpha p180 subunit antibody
      • DPOLA_HUMAN antibody
      • OTTHUMP00000023089 antibody
      • OTTHUMP00000023090 antibody
      • P180 antibody
      • p180 subunit antibody
      • POLA antibody
      • POLA1 antibody
      • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
      • Polymerase (DNA-directed), alpha (70kD) antibody
      see all

    Anti-DNA polymerase alpha antibody 图像



    • Predicted band size : 166 kDa
      DNA polymerase alpha antibody (ab57296) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-DNA polymerase alpha antibody (ab57296)参考文献

    ab57296 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email.
    I am sending you the discount code for trying the antibody on mouse samples - a species we have not yet tried.

    I will forward your email to xxx so that she can complete your order with ab57296.
    Please let me k...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"