概述

  • 产品名称Anti-DNA PKcs抗体
    参阅全部 DNA PKcs 一抗
  • 描述
    兔多克隆抗体to DNA PKcs
  • 特异性ab53701 detects endogenous levels of total DNA PKcs protein.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human DNA PKcs. Immunogen range = 4061-4110
    Database link: P78527

  • 阳性对照
    • Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab53701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 469 kDa. PubMed: 26065416

靶标

  • 功能Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
  • 序列相似性Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
    Contains 3 TPR repeats.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
    S-nitrosylated by GAPDH.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA dependent protein kinase catalytic subunit antibody
    • DNA PK catalytic subunit antibody
    • DNA-dependent protein kinase catalytic subunit antibody
    • DNA-PK catalytic subunit antibody
    • DNA-PKcs antibody
    • DNAPK antibody
    • DNAPK catalytic subunit antibody
    • DNPK 1 antibody
    • DNPK1 antibody
    • Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
    • HYRC 1 antibody
    • HYRC antibody
    • HYRC1 antibody
    • IMD26 antibody
    • p350 antibody
    • p460 antibody
    • PKRDC antibody
    • PRKDC antibody
    • PRKDC_HUMAN antibody
    • Protein Kinase DNA Activated Catalytic Polypeptide antibody
    • XRCC 7 antibody
    • XRCC7 antibody
    see all

Anti-DNA PKcs antibody 图像

  • ab53701 at 1/50 dilution staining DNA PKcs in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • ab53701 at 1/50 dilution staining DNA PKs in human FFPE Hela cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 15 minutes at room temperature and then incubated with ab53701 for 1 hour. A donkey polycolnal conjugated with HRP was used as the secondary antibody.

    See Abreview

Anti-DNA PKcs antibody (ab53701)参考文献

This product has been referenced in:
  • Burdine LJ  et al. Proteomic Identification of DNA-PK Involvement within the RET Signaling Pathway. PLoS One 10:e0127943 (2015). WB ; Human . Read more (PubMed: 26065416) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (FFPE Hela cell line)
Specification FFPE Hela cell line
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Low pH buffer
Permeabilization No
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"