使用敲除细胞株进行验证

Anti-DNA PKcs抗体[18-2] (ab44815)

概述

  • 产品名称Anti-DNA PKcs抗体[18-2]
    参阅全部 DNA PKcs 一抗
  • 描述
    小鼠单克隆抗体[18-2] to DNA PKcs
  • 经测试应用适用于: Inhibition Assay, ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Other Immunogen Type corresponding to Human DNA PKcs aa 1-2713. Immunogen is purified from HeLa cells.
    Database link: P78527

  • 表位amino acids 1-2713
  • 阳性对照
    • LS174T cells. Tonsil.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab44815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Inhibition Assay
ICC/IF
IP
WB
IHC-P
  • 应用说明ICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/400.
    IP: 1/400.
    Inhib (Inhibits (50%) DNA-dependent Phosphorylation): Use at an assay dependent dilution.
    WB: 1/200. Predicted molecular weight: 469 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
    • 序列相似性Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
      Contains 3 TPR repeats.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
      S-nitrosylated by GAPDH.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA dependent protein kinase catalytic subunit antibody
      • DNA PK catalytic subunit antibody
      • DNA-dependent protein kinase catalytic subunit antibody
      • DNA-PK catalytic subunit antibody
      • DNA-PKcs antibody
      • DNAPK antibody
      • DNAPK catalytic subunit antibody
      • DNPK 1 antibody
      • DNPK1 antibody
      • Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
      • HYRC 1 antibody
      • HYRC antibody
      • HYRC1 antibody
      • IMD26 antibody
      • p350 antibody
      • p460 antibody
      • PKRDC antibody
      • PRKDC antibody
      • PRKDC_HUMAN antibody
      • Protein Kinase DNA Activated Catalytic Polypeptide antibody
      • XRCC 7 antibody
      • XRCC7 antibody
      see all

    Anti-DNA PKcs antibody [18-2] 图像



    • Predicted band size : 469 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: DNA PKcs knockout HAP1 cell lysate (40 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: SHSY5Y cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab44815 observed at 460 kDa. Red - loading control, ab18251, observed at 52 kDa.

      ab44815 was shown to specifically react with DNA PKcs when DNA PKcs knockout samples were used. Wild-type and DNA PKcs knockout samples were subjected to SDS-PAGE. Ab44815 and ab18251 (loading control to alpha tubulin) were diluted at 1/200 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    Anti-DNA PKcs antibody [18-2] (ab44815)参考文献

    ab44815 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step 0.25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
    Specification mouse embryonic spinal cord E11
    Permeabilization Yes - 0.25%TritonX100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Aug 07 2014

    Thank you for contacting us regarding this. I've spoken with the lab regarding our Anti-DNA PKcs antibodies and have found that  ab1832 would most likely fulfill your needs as it's epitope site is between  aa1-2713. I hope this information is helpful t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"