The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
Use a concentration of 1 µg/ml.
功能Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
组织特异性Testis, thymus, prostate and heart.
疾病相关LIG4 syndrome Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation
序列相似性Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.
All lanes : Anti-DNA Ligase IV antibody (ab65130) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells Lane 2 : Extracts from Jurkat cells, with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 104 kDa Observed band size : 104 kDa Additional bands at : 28 kDa,48-55 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence-DNA Ligase IV antibody(ab65130)
ICC/IF image of ab65130 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-DNA Ligase IV antibody (ab65130)参考文献
has not yet been referenced specifically in any publications.