Anti-DNA Ligase IV抗体(ab65130)


  • 产品名称Anti-DNA Ligase IV抗体
    参阅全部 DNA Ligase IV 一抗
  • 描述
    兔多克隆抗体to DNA Ligase IV
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthesized peptide derived from an internal region of human DNA Ligase IV

  • 阳性对照
    • Extracts from Jurkat cells


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab65130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • 组织特异性Testis, thymus, prostate and heart.
  • 疾病相关LIG4 syndrome
    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation
  • 序列相似性Belongs to the ATP-dependent DNA ligase family.
    Contains 2 BRCT domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA ligase IV ATP dependent antibody
    • DNA joinase antibody
    • DNA ligase 4 antibody
    • DNA ligase IV antibody
    • DNA repair enzyme antibody
    • DNLI4_HUMAN antibody
    • LIG 4 antibody
    • LIG4 antibody
    • LIG4S antibody
    • Ligase IV antibody
    • Ligase IV DNA ATP dependent antibody
    • Polydeoxyribonucleotide synthase [ATP] 4 antibody
    • Polydeoxyribonucleotide synthase 4 antibody
    • Polydeoxyribonucleotide synthase antibody
    • Polynucleotide ligase antibody
    • Sealase antibody
    see all

Anti-DNA Ligase IV antibody 图像

  • All lanes : Anti-DNA Ligase IV antibody (ab65130) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells, with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 104 kDa
    Observed band size : 104 kDa
    Additional bands at : 28 kDa,48-55 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65130 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DNA Ligase IV antibody (ab65130)参考文献

ab65130 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65130.
Please use the links above to contact us or submit feedback about this product.