概述

  • 产品名称Anti-DNA Ligase I抗体
    参阅全部 DNA Ligase I 一抗
  • 描述
    兔多克隆抗体to DNA Ligase I
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region of Human DNA Ligase I (NP_000225)

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab76232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 102 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

靶标

Anti-DNA Ligase I antibody 图像

  • Anti-DNA Ligase I antibody (ab76232) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-DNA Ligase I antibody (ab76232)参考文献

This product has been referenced in:
  • Lokanga RA  et al. Heterozygosity for a hypomorphic Polß mutation reduces the expansion frequency in a mouse model of the Fragile X-related disorders. PLoS Genet 11:e1005181 (2015). WB ; Mouse . Read more (PubMed: 25886163) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"