Anti-DNA Gyrase B抗体(ab75596)

概述

  • 产品名称Anti-DNA Gyrase B抗体
    参阅全部 DNA Gyrase B 一抗
  • 描述
    兔多克隆抗体to DNA Gyrase B
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli , Staphylococcus aureus
  • 免疫原

    Purified E. coli Gyr B protein (over expressed from E. coli)

  • 阳性对照
    • E. coli cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明This antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000.

Can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed gyrase in cell extracts. The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性DNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
  • 数据库链接
    • 别名
      • acrB antibody
      • DNA gyrase subunit B antibody
      • himB antibody
      • hisU antibody
      • nalC antibody
      • parA antibody
      • pcbA antibody
      see all

    Anti-DNA Gyrase B antibody 图像

    • All lanes : Anti-DNA Gyrase B antibody (ab75596) at 1/1000 dilution

      Lane 1 : E. coli cell lysate (Gyrase A, whole protein) at 0.1 µg
      Lane 2 : E. coli cell lysate (Gyrase A, whole protein) at 0.5 µg
      Lane 3 : E. coli cell lysate (Gyrase A, whole protein) at 1 µg
      Lane 4 : E. coli cell lysate (Gyrase B, whole protein) at 0.1 µg
      Lane 5 : E. coli cell lysate (Gyrase B, whole protein) at 0.5 µg
      Lane 6 : E. coli cell lysate (Gyrase B, whole protein) at 1 µg
      Lane 7 : E. coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
      Lane 8 : E. coli cell lysate (Gyrase A, A59 domain) at 0.1 µg

      Secondary
      goat anti rabbit Igs at 1/5000 dilution

      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Anti-DNA Gyrase B antibody (ab75596)参考文献

    ab75596 has not yet been referenced specifically in any publications.

    Product Wall



    This polyclonal antibody ab75596 it will recognise all gyrase/fragments whole protein or separate domains.
    If you wish to use a more specific antibody which recognises the gyrase B43 and 24 domains, we carry the monclonal antibody [7D3] w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"