Anti-DNA Gyrase B抗体[7D3] (ab75586)

概述

  • 产品名称Anti-DNA Gyrase B抗体[7D3]
    参阅全部 DNA Gyrase B 一抗
  • 描述
    小鼠单克隆抗体[7D3] to DNA Gyrase B
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Purified Escherichia coli DNA Gyrase B protein (over expressed from Escherichia coli)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified from tissue culture supernatant on a Protein A column using the low salt method. The antibody is purified to >95% purity as judged by SDS polyacrylamide gel electrophoresis.
  • 克隆单克隆
  • 克隆编号7D3
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1000. The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts. It can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed Gyrase in cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
    • 数据库链接
      • 别名
        • acrB antibody
        • DNA gyrase subunit B antibody
        • himB antibody
        • hisU antibody
        • nalC antibody
        • parA antibody
        • pcbA antibody
        see all

      Anti-DNA Gyrase B antibody [7D3] 图像

      • All lanes : Anti-DNA Gyrase B antibody [7D3] (ab75586) at 1/1000 dilution

        Lane 1 : E. coli cell lysate (B24 domain of DNA Gyrase B protein)
        Lane 2 : E. coli cell lysate (B43 domain of DNA Gyrase B protein)
        Lane 3 : E. coli cell lysate (whole DNA Gyrase B protein)

        Lysates/proteins at 200 µg per lane.

        Secondary
        HRP labelled rabbit anti mouse IgGs at 1/5000 dilution

        Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      Anti-DNA Gyrase B antibody [7D3] (ab75586)参考文献

      ab75586 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"