概述

  • 产品名称Anti-DNA Gyrase A抗体
    参阅全部 DNA Gyrase A 一抗
  • 描述
    兔多克隆抗体to DNA Gyrase A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli , Staphylococcus aureus
  • 免疫原

    Purified E. coli DNA Gyrase A protein (over expressed from E. coli)

  • 阳性对照
    • E.coli cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明The antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed gyrase in cell extracts.
    The antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • 数据库链接
      • 别名
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody 图像

      • All lanes : Anti-DNA Gyrase A antibody (ab75594) at 1/1000 dilution

        Lane 1 : E coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
        Lane 2 : E coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
        Lane 3 : E coli cell lysate (Gyrase B, whole protein) at 1 µg
        Lane 4 : E coli cell lysate (Gyrase B, whole protein) at 0.5 µg
        Lane 5 : E coli cell lysate (Gyrase B, whole protein) at 0.1 µg
        Lane 6 : E coli cell lysate (Gyrase A, whole protein) at 1 µg
        Lane 7 : E coli cell lysate (Gyrase A, whole protein) at 0.5 µg
        Lane 8 : E coli cell lysate (Gyrase A, whole protein) at 0.1 µg

        Secondary
        goat anti rabbit Igs at 1/5000 dilution

        Observed band size : 97 kDa (why is the actual band size different from the predicted?)

      Anti-DNA Gyrase A antibody (ab75594)参考文献

      This product has been referenced in:
      • Aedo S & Tse-Dinh YC Isolation and quantitation of topoisomerase complexes accumulated on escherichia coli chromosomal DNA. Antimicrob Agents Chemother 56:5458-64 (2012). WB . Read more (PubMed: 22869559) »

      See 1 Publication for this product

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      Most of our antibodies are stable at -20C for up to five years. For short term storage we recommend storing at 4C. It is also important to aliquot them in order to minimize damage due to freezing and thawing.
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      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"