Anti-DNA Gyrase A抗体[7F11] (ab75592)

概述

  • 产品名称Anti-DNA Gyrase A抗体[7F11]
    参阅全部 DNA Gyrase A 一抗
  • 描述
    小鼠单克隆抗体[7F11] to DNA Gyrase A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Full length purified E.coli DNA Gyrase A protein (over-expressed from E.coli)

  • 表位N-terminal domain of DNA Gyrase A.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明ab75592 is purified on a Protein A column using the low-salt method. It is purified to >95% purity as judged by SDS-polyacrylamide gel electrophoresis.
  • 克隆单克隆
  • 克隆编号7F11
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/200 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
    Can be used in Western Blots to detect purified over-expressed DNA Gyrase A and DNA Gyrase A in cell extracts.
    Primary antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • 数据库链接
      • 别名
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody [7F11] 图像

      • All lanes : Anti-DNA Gyrase A antibody [7F11] (ab75592) at 1/1000 dilution

        Lane 1 : Gyrase B, B24 domain
        Lane 2 : Gyrase B, B43 domain
        Lane 3 : Gyrase B, whole protein
        Lane 4 : Gyrase A, A59 domain
        Lane 5 : Gyrase A, whole protein

        Secondary
        HRP labelled rabbit anti-mouse IgGs at 1/5000 dilution

        Predicted band size : 97 kDa
        Observed band size : 97 kDa

      Anti-DNA Gyrase A antibody [7F11] (ab75592)参考文献

      ab75592 has not yet been referenced specifically in any publications.

      Product Wall

      Thank you for your enquiry. ab75592 and ab75593 are monclonal antibodies specific to different E coli gyrase protein domains so I'm not sure whether they will react. We haven't tested them against S. aureus gyrase. We have two polyclonal antibodies ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"