Anti-DNA Gyrase A抗体[4D3] (ab75585)


  • 产品名称Anti-DNA Gyrase A抗体[4D3]
    参阅全部 DNA Gyrase A 一抗
  • 描述
    小鼠单克隆抗体[4D3] to DNA Gyrase A
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Escherichia coli
  • 免疫原

    Purified E. coli DNA Gyrase A protein (over expressed from E. coli)

  • 阳性对照
    • E. coli cell lysate


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified from tissue culture supernatant on a Protein A column using the low salt method. The antibody is purified to >95% purity as judged by SDS polyacrylamide gel electrophoresis.
  • 克隆单克隆
  • 克隆编号4D3
  • 同种型IgG2b
  • 研究领域


Our Abpromise guarantee covers the use of ab75585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed Gyrase in cell extracts.
    Primary antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • 数据库链接
      • 别名
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody [4D3] 图像

      • All lanes : Anti-DNA Gyrase A antibody [4D3] (ab75585) at 1/1000 dilution

        Lane 1 : B24 (a domain of the DNA Gyrase B protein)
        Lane 2 : B43 (a domain of the DNA Gyrase B protein)
        Lane 3 : B (the whole DNA Gyrase B protein)
        Lane 4 : A59 (a domain of the DNA Gyrase A protein)
        Lane 5 : A (the whole DNA Gyrase A protein)

        Lysates/proteins at 200 µg per lane.

        HRP labelled rabbit anti mouse IgG at 1/5000 dilution

        Observed band size : 97 kDa (why is the actual band size different from the predicted?)

      Anti-DNA Gyrase A antibody [4D3] (ab75585)参考文献

      ab75585 has not yet been referenced specifically in any publications.

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