DNA Damage Kinases组合(ATM, ATM phospho S1981, DNA-PKcs, DNA-PKcs phospho S2056) (ab103970)

概述

  • 产品名称DNA Damage Kinases组合(ATM, ATM phospho S1981, DNA-PKcs, DNA-PKcs phospho S2056)
  • 产品概述

    ab103970 is a DNA Damage Kinases Panel designed for the validation and characterization of the phosphorylation state of the DNA damage responsive kinases ATM and DNA-PKcs. ATM and DNA-PK are protein kinases that are active and become phosphorylated in response to DNA double strand breaks (DSBs). ATM is phosphorylated on S1981 and phosphorylates various proteins during the cellular response to DNA DSBs, while DNA-PK, a key component of the non-homologous end-joining pathway of DSB repair, is phosphorylated on S2056.

性能

  • 别名
    • A-T mutated
    • A-T mutated homolog
    • Ataxia telangiectasia mutated
    • ATM
    • ATM_HUMAN
    • DNA dependent protein kinase catalytic subunit
    • DNA PK
    • DNA PK catalytic subunit
    • DNA PKcs
    • DNA-dependent protein kinase catalytic subunit
    • DNA-PK catalytic subunit
    • DNA-PKcs
    • DNAPK
    • DNPK1
    • p460
    • PRKDC
    • PRKDC_HUMAN
    • Serine protein kinase ATM
    • Serine-protein kinase ATM
    • Serine/threonine-protein kinase ATM
    • TEL1
    • XRCC 7
    • XRCC7
    see all

DNA Damage Kinases Panel (ATM, ATM phospho S1981, DNA-PKcs, DNA-PKcs phospho S2056) 图像

  • All lanes : Anti-ATM antibody (ab82512) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    ab82512 was raised against an immunogen that is predicted to cross react with both isoform 1 (350 kDa) and isoform 2 (195 kDa) of Human Serine-protein kinase ATM (ATM). We believe that the band observed at 195 kDa corresponds to isoform 2.
  • All lanes : Anti-ATM (phospho S1981) antibody [10H11.E12] (ab36810) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Extract from Patient with Ataxia-Telangiectasia Whole Cell Lysate
    Lane 3 : Irradiated HeLa Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Additional bands at : 100 kDa,110 kDa,145 kDa,200 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-DNA PKcs antibody (ab70250) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 15 µg
    Lane 2 : Whole cell lysate from Hela cells at 50 µg


    Additional bands at : 170 kDa,180 kDa,230 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-DNA PKcs (phospho S2056) antibody - ChIP Grade (ab18192) at 1 µg/ml

    Lane 1 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823)
    Lane 2 : Untreated HeLa cell extract
    Lane 3 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
    Lane 4 : Untreated HeLa cell extract with Human DNA PKcs (phospho S2056) peptide (ab20406) at 1 µg/ml
    Lane 5 : HeLa Gamma Irradiated Whole Cell Lysate Pack (ab13823) with Human DNA PKcs peptide (ab20407) at 1 µg/ml
    Lane 6 : Untreated HeLa cell extract with Human DNA PKcs peptide (ab20407) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
    ab18192 specifically recognizes a band at ~460 kDa corresponding to DNA PKcs in HeLa cells that have been treated with ionizing radiation (lane 1). This band is not detected in untreated cells (lane 2). The activity of the antibody is quenched by the addition of the immunizing (modified) peptide, ab20406 (lanes 3) but not the unmodified peptide, ab20407 (lane 5). For the ab13823 irradiated HeLa cell lysate, the 4 hour post-treatment extract was used.

DNA Damage Kinases Panel (ATM, ATM phospho S1981, DNA-PKcs, DNA-PKcs phospho S2056) (ab103970)参考文献

This product has been referenced in:
  • Velkova A  et al. Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle 9:1421-33 (2010). Read more (PubMed: 20305393) »
  • Sakasai R  et al. Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin. DNA Repair (Amst) 9:76-82 (2010). Read more (PubMed: 19959400) »

See all 2 Publications for this product

Product Wall

The kinase panel (ab103970) consists for 4 primary antibodies, immunogen information below:

ab18192
Synthetic peptide conjugated to KLH derived from within residues 2050 - 2150 of Human DNA PKcs. (Note: the amino acid sequence is propri...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"