概述

  • 产品名称Anti-DMT1抗体
    参阅全部 DMT1 一抗
  • 描述
    小鼠单克隆抗体to DMT1
  • 经测试应用适用于: Flow Cyt, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment: MVLGPEQKMSDDSVSGDHGESASLGNINPYSNPSLSQSPGDSEEYFATYF NEKISIPEEEYSCF, corresponding to amino acids 1-66 of Human DMT1

性能

应用

Our Abpromise guarantee covers the use of ab55735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-Fr 1/1000.

靶标

  • 功能Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
  • 组织特异性Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
  • 疾病相关Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels.
  • 序列相似性Belongs to the NRAMP family.
  • 翻译后修饰Ubiquitinated by WWP2.
  • 细胞定位Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCT 1 antibody
    • dct-1 antibody
    • DCT1 antibody
    • Divalent cation transporter 1 antibody
    • Divalent metal transporter 1 antibody
    • DMT 1 antibody
    • DMT-1 antibody
    • DMT1 antibody
    • FLJ37416 antibody
    • Natural resistance associated macrophage protein 2 antibody
    • Natural resistance-associated macrophage protein 2 antibody
    • NRAM2_HUMAN antibody
    • NRAMP 2 antibody
    • NRAMP2 antibody
    • OK/SW-cl.20 antibody
    • Slc11a2 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody
    • Solute carrier family 11 member 2 antibody
    see all

Anti-DMT1 antibody 图像

  • IHC-Fr image of DMT1 staining on mouse duodenum using ab55735 (1:1000). The sections were fixed with paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were blocked using 10 % donkey serum for 1 hour at 24°C. ab55735 was diluted 1:1000 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and incubated with the slides at 4°C for 24 hours. The secondary antibody used was donkey polyclonal against mouse IgG conjugated to Alexa Fluor 568 (1:1000)

    See Abreview

  • DMT1 antibody (ab55735) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.
  • Western blot against tagged recombinant protein immunogen using ab55735 DMT1 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab55735 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55735, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-DMT1 antibody (ab55735)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Mouse duodenum)
Specification Mouse duodenum
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jul 16 2013

Thank you for your email.

We have monoclonal antibody ab55735 but it not tested in ICC/IF. http://www.abcam.com/DMT1-antibody-ab55735.html

http://www.abcam.com/DMT1-antibody-4C6-ab117544.html

I will be pleased to offer you te...

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Thank you for contacting us. Ab55735 will recognize all 4 isotypes of this protein in human as will ab123085. I've noticed that the isoforms differ at the C-terminal end. The immunogens for Ab55812 and ab117544 are against the N-terminal region of ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this DMT1 antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"