Anti-DMT1抗体(ab123085)

概述

  • 产品名称Anti-DMT1抗体
    参阅全部 DMT1 一抗
  • 描述
    兔多克隆抗体to DMT1
  • 经测试应用适用于: ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 261-291 of Human DMT1 (NP_001167599.1, NP_000608.1, NP_001167597.1, NP_001167600.1).

  • 阳性对照
    • Mouse cerebellum tissue lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab123085 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab123085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
IHC-FoFr 1/100.
WB 1/100 - 1/500. Predicted molecular weight: 62 kDa.

靶标

  • 功能Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
  • 组织特异性Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
  • 疾病相关Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels.
  • 序列相似性Belongs to the NRAMP family.
  • 翻译后修饰Ubiquitinated by WWP2.
  • 细胞定位Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCT 1 antibody
    • dct-1 antibody
    • DCT1 antibody
    • Divalent cation transporter 1 antibody
    • Divalent metal transporter 1 antibody
    • DMT 1 antibody
    • DMT-1 antibody
    • DMT1 antibody
    • FLJ37416 antibody
    • Natural resistance associated macrophage protein 2 antibody
    • Natural resistance-associated macrophage protein 2 antibody
    • NRAM2_HUMAN antibody
    • NRAMP 2 antibody
    • NRAMP2 antibody
    • OK/SW-cl.20 antibody
    • Slc11a2 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody
    • Solute carrier family 11 member 2 antibody
    see all

Anti-DMT1 antibody 图像

  • ICC/IF image of DMT1 staining on Caco2 cells using ab123085 (1:100). The cells were fixed using paraformaldehyde and permeabilized using 0.1%TritonX in 0.1 %PBS. The cells were blocked using 10% donkey serum for 1 hour at 24°C. ab123085 (1:100) was incubated with the cells for 4 hours at 25°C. The secondary antibody used was donkey polyclonal to Rabbit IgG conjugated to Alexa Fluor 488.

    See Abreview

  • Anti-DMT1 antibody (ab123085) at 1/1000 dilution + Mouse cerebellum tissue lysates at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 62 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • IHC-FoFr image of DMT1 staining on mouse intestine tissue using ab123085 (1:100). Adult mouse was perfused with 4% PFA in 0.1M PBS. The sections were cut using a cryostat and permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using donkey serum for 1 hour at 24°C. ab123085 (1:100) was incubated with the sections for 24 hours at 4°C. The secondary antibody used was donkey polyclonal to mouse IgG conjugated to alexa fluor 488 (1:1000).

    See Abreview

Anti-DMT1 antibody (ab123085)参考文献

ab123085 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human duodenal cell line)
Specification Human duodenal cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Duodenum)
Specification Duodenum
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Feb 14 2013

Thank you for contacting us. Ab55735 will recognize all 4 isotypes of this protein in human as will ab123085. I've noticed that the isoforms differ at the C-terminal end. The immunogens for Ab55812 and ab117544 are against the N-terminal region of ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"