Anti-DMT1抗体[4C6] (ab117544)

概述

  • 产品名称Anti-DMT1抗体[4C6]
    参阅全部 DMT1 一抗
  • 描述
    小鼠单克隆抗体[4C6] to DMT1
  • 经测试应用适用于: WB, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human DMT1 protein (NP_000608.1).

  • 阳性对照
    • Human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号4C6
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab117544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.
ICC/IF 1/1000.

靶标

  • 功能Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
  • 组织特异性Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
  • 疾病相关Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels.
  • 序列相似性Belongs to the NRAMP family.
  • 翻译后修饰Ubiquitinated by WWP2.
  • 细胞定位Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DCT 1 antibody
    • dct-1 antibody
    • DCT1 antibody
    • Divalent cation transporter 1 antibody
    • Divalent metal transporter 1 antibody
    • DMT 1 antibody
    • DMT-1 antibody
    • DMT1 antibody
    • FLJ37416 antibody
    • Natural resistance associated macrophage protein 2 antibody
    • Natural resistance-associated macrophage protein 2 antibody
    • NRAM2_HUMAN antibody
    • NRAMP 2 antibody
    • NRAMP2 antibody
    • OK/SW-cl.20 antibody
    • Slc11a2 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 2 antibody
    • Solute carrier family 11 member 2 antibody
    see all

Anti-DMT1 antibody [4C6] 图像

  • ICC/IF image of DMT1 staining on Caco2 cells using ab117544 (1:1000). The cells were fixed with paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were blocked using 10 % donkey serum for 1 hour at 24°C. ab117544 was diluted 1:1000 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and incubated with the slides at 4°C for 24 hours. The secondary antibody used was donkey polyclonal against mouse IgG conjugated to Alexa Fluor 488 (undiluted)

    See Abreview

  • ab117544, at 5 µg/ml, staining DMT1 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ELISA Image: the detection limit for recombinant proprietary tagged DMT1 is 0.03 ng/ml as a capture antibody.

Anti-DMT1 antibody [4C6] (ab117544)参考文献

ab117544 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (CaCo2 cell)
Specification CaCo2 cell
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jul 16 2013

Thank you for your email.

We have monoclonal antibody ab55735 but it not tested in ICC/IF. http://www.abcam.com/DMT1-antibody-ab55735.html

http://www.abcam.com/DMT1-antibody-4C6-ab117544.html

I will be pleased to offer you te...

Read More

Thank you for contacting us. Ab55735 will recognize all 4 isotypes of this protein in human as will ab123085. I've noticed that the isoforms differ at the C-terminal end. The immunogens for Ab55812 and ab117544 are against the N-terminal region of ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"