Anti-DMRT3抗体(ab107043)

概述

  • 产品名称Anti-DMRT3抗体
    参阅全部 DMRT3 一抗
  • 描述
    兔多克隆抗体to DMRT3
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 438-467 of Human DMRT3 (NP_067063.1), conjugated to KLH.

  • 阳性对照
    • Mouse kidney tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab107043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 51 kDa.
IHC-Fr 1/100.

靶标

Anti-DMRT3 antibody 图像

  • IHC-Fr image of DMRT3 staining on zebrafish retina sections using ab107043 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab107043 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • Anti-DMRT3 antibody (ab107043) at 1/100 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 51 kDa

Anti-DMRT3 antibody (ab107043)参考文献

ab107043 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retinal Neurons, forebrain neurons)
Specification Retinal Neurons, forebrain neurons
Permeabilization Yes - triton X
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

提交于 Aug 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"