概述

  • 产品名称Anti-DMPO Nitrone Adduct抗体
    参阅全部 DMPO Nitrone Adduct 一抗
  • 描述
    兔多克隆抗体to DMPO Nitrone Adduct
  • 经测试应用适用于: WB, ELISAmore details
  • 免疫原

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.

  • 常规说明


    Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.

性能

应用

Our Abpromise guarantee covers the use of ab23702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa.
ELISA 1/1000 - 1/5000.

靶标

  • 相关性For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • 别名
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Anti-DMPO Nitrone Adduct antibody 图像

  • All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)

    Lane 1 : Metmyoglobin at 3 µl
    Lane 2 : Metmyoglobin-DMPO at 3 µl
    Lane 3 : Metmyoglobin-DMPO at 5 µl
    Lane 4 : Metmyoglobin-DMPO at 10 µl


    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

Anti-DMPO Nitrone Adduct antibody (ab23702)参考文献

This product has been referenced in:
  • Gomez-Mejiba SE  et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . Read more (PubMed: 19159679) »
  • Schildknecht S  et al. Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite. FASEB J 22:215-24 (2008). WB . Read more (PubMed: 17724253) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"