概述

  • 产品名称
    Anti-DMPO Nitrone Adduct抗体
    参阅全部 DMPO Nitrone Adduct 一抗
  • 描述
    兔多克隆抗体to DMPO Nitrone Adduct
  • 经测试应用
    适用于: WB, ELISAmore details
  • 免疫原

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.

  • 常规说明


    Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.

性能

应用

Our Abpromise guarantee covers the use of ab23702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa.
ELISA 1/1000 - 1/5000.

靶标

  • 相关性
    For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • 别名
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

图片

  • All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)

    Lane 1 : Metmyoglobin at 3 µl
    Lane 2 : Metmyoglobin-DMPO at 3 µl
    Lane 3 : Metmyoglobin-DMPO at 5 µl
    Lane 4 : Metmyoglobin-DMPO at 10 µl


    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Pathak V  et al. Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II. PLoS One 12:e0181732 (2017). WB ; Spinacia oleracea . Read more (PubMed: 28732060) »
  • Gomez-Mejiba SE  et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . Read more (PubMed: 19159679) »

See all 4 Publications for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab23702.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册