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Our Abpromise guarantee covers the use of ab82351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa).|
ab82351 staining DMP1 in human tooth tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with methanol and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.