概述

  • 产品名称Anti-DMP1抗体
    参阅全部 DMP1 一抗
  • 描述
    小鼠单克隆抗体to DMP1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment, corresponding to amino acids 221-331 of Human DMP1 (NP_004398) with tag.

  • 阳性对照
    • Recombinant tagged human DMP1 fragment.

性能

应用

Our Abpromise guarantee covers the use of ab76632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性DMP1 is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. It is critical for proper mineralization of bone and dentin, and is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. DMP1 may also have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts the unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
    • 细胞定位Nucleus. Cytoplasm. Secreted; extracellular space; extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
    • 数据库链接
    • 别名
      • ARHP antibody
      • ARHR antibody
      • AV020965 antibody
      • Dentin matrix acidic phosphoprotein 1 antibody
      • Dentin matrix protein 1 antibody
      • DENTMAT antibody
      • DMP 1 antibody
      • Dmp antibody
      • MGC130441 antibody
      • PP antibody
      • Serine rich acidic phosphoprotein antibody
      see all

    Anti-DMP1 antibody 图像

    • Anti-DMP1 antibody (ab76632) at 1 µg/ml + Recombinant tagged human DMP1 fragment at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 56 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    Anti-DMP1 antibody (ab76632)参考文献

    This product has been referenced in:
    • Pilia M  et al. Influence of substrate curvature on osteoblast orientation and extracellular matrix deposition. J Biol Eng 7:23 (2013). Human . Read more (PubMed: 24090183) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab76632.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"