Anti-DMC1抗体[2H12/4] (ab11054)
Key features and details
- Mouse monoclonal [2H12/4] to DMC1
- Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
概述
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产品名称
Anti-DMC1抗体[2H12/4]
参阅全部 DMC1 一抗 -
描述
小鼠单克隆抗体[2H12/4] to DMC1 -
宿主
Mouse -
特异性
Specific for DMC1 - does not cross-react with the related protein Rad51. The antibody was tested on mouse meiotic chromosomes. It allowed localisation of the Dmc1 protein as numerous foci in the Leptotene and Zygotene stages of meiotic prophase, and fewer foci in the early Pachytene stage of meiotic prophase. -
经测试应用
适用于: IP, WB, IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Recombinant fusion protein (Human).
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阳性对照
- ICC:mouse meiotic chromosomes
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
2H12/4 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11054于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | (1) |
Use at an assay dependent concentration.
Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
说明 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
靶标
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功能
May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks. -
序列相似性
Belongs to the RecA family. DMC1 subfamily.
Contains 1 HhH domain. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 618398 Cow
- Entrez Gene: 11144 Human
- Entrez Gene: 13404 Mouse
- Entrez Gene: 362960 Rat
- Omim: 602721 Human
- SwissProt: Q14565 Human
- SwissProt: Q61880 Mouse
- Unigene: 339396 Human
see all -
别名
- Disrupted meiotic cDNA 1 homolog antibody
- Disrupted meiotic cDNA 1, yeast, homolog of antibody
- dJ199H16.1 antibody
see all
图片
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Overlay histogram showing HeLa cells stained with ab11054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab11054 被引用在 24 文献中.
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Chen Y et al. The SUN1-SPDYA interaction plays an essential role in meiosis prophase I. Nat Commun 12:3176 (2021). PubMed: 34039995
- Ghouil R et al. BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination. Nat Commun 12:4605 (2021). PubMed: 34326328
- Hinch AG et al. The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates. Mol Cell 79:689-701.e10 (2020). PubMed: 32610038
- Hutka M et al. Exogenous Gonadotrophin Stimulation Induces Partial Maturation of Human Sertoli Cells in a Testicular Xenotransplantation Model for Fertility Preservation. J Clin Med 9:N/A (2020). PubMed: 31963729