Anti-DMAP1抗体- ChIP Grade (ab2848)

概述

  • 产品名称Anti-DMAP1抗体- ChIP Grade
    参阅全部 DMAP1 一抗
  • 描述
    兔多克隆抗体to DMAP1 - ChIP Grade
  • 特异性Detects DNA methyltransferase 1 associated protein (DMAP1).
  • 经测试应用适用于: ChIP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Mouse DMAP1 aa 435-450.
    Sequence:

    DTIIDVVGAPLTRNSR


    (Peptide available as ab4919)

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明

     

     

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. DNA methyltransferase 1 associated protein (DMAP1) has been shown to interact directly with the N-terminus of DNA Methyltransferase 1 (Dnmt1) throughout DNA replication during S phase. DMAP1 has also been identified as a critical gene product in myotonic dystrophy.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab2848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 20864525
IHC-P 1/20 - 1/200.
WB 1/500. Predicted molecular weight: 52.9 kDa.Can be blocked with DMAP1 peptide (ab4919).
ICC/IF 1/1000.

靶标

  • 相关性DMAP1 is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DKFZp686L09142 antibody
    • DMAP 1 antibody
    • DNA methyltransferase 1 associated protein 1 antibody
    • DNMAP 1 antibody
    • DNMAP1 antibody
    • DNMT1 associated protein 1 antibody
    • DNMTAP 1 antibody
    • DNMTAP1 antibody
    • EAF 2 antibody
    • EAF2 antibody
    • FLJ11543 antibody
    • KIAA1425 antibody
    • MAT 1 mediated transcriptional repressor antibody
    • MGC55593 antibody
    • SWC 4 antibody
    • SWC4 antibody
    • zgc:55593 antibody
    see all

Anti-DMAP1 antibody - ChIP Grade 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated human esophageal carcinoma tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2848 diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2848 diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated mouse uterus tissue (right) compared with a negative control in the absence of primary antibody (left). Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then incubated with ab2848 diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • All lanes : Anti-DMAP1 antibody - ChIP Grade (ab2848) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Mouse brain cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 52.9 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • ab2848 staining DMAP1 in asynchronous HeLa cells in conjunction with a secondary goat anti-rabbit antibody conjugated to Cy3 ® (green). Cells were counterstained with DAPI (red) to show the nucleus. Please refer to abreview for further details.

    See Abreview



  • Predicted band size : 52.9 kDa

    Image from Lee GE, et al, J Biol Chem. 2010 Nov 26;285(48):37630-40. Epub 2010 Sep 23, Fig 2.

    The in vivo activity of endogenous DNMT1 was measured in HeLa cells infected with shRNA expressing lentivirus 2 days post-infection. The cells were pulsed with 10 µm of aza-dC for 1 hour and rapidly lysed with 1% sarkosyl followed by CsCl step gradient purification of genomic DNA. DNA fractions (1.7g/cc) were pooled, and the DNA concentration was measured by absorbance at 260 nm. Either 0.5, 1, or 2 µg of genomic DNA were applied to nitrocellulose membrane using the slot blot manifold. To analyze the protein expression, nuclear proteins were extracted. The cells were harvested and washed twice with a hypotonic buffer (10 mm Hepes, pH 7.6, 1.5 mm MgCl2, 10 mm KCl, 1 mm DTT) followed by a 10-minute incubation with hypotonic buffer on ice. The cells were homogenized by 10 strokes with a tight fitting Dounce homogenizer, and the nuclei were collected with centrifugation at 13,000 rpm, 4 °C for 30 seconds. The nuclei were then suspended in a salt extraction buffer (20 mm Hepes, pH 7.6,
  • Anti-DMAP1 antibody - ChIP Grade (ab2848) at 1/500 dilution + whole cell lysate prepared from Xenopus laevis egg at 10 µg

    Secondary
    HRP-Protein A at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 52.9 kDa


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-DMAP1 antibody - ChIP Grade (ab2848)参考文献

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T, MEL, MCF7, K562)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 20 µg
Specification 293T, MEL, MCF7, K562
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Nov 17 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Human Tissue sections (human duodenum)
Specification human duodenum
Permeabilization Yes - tween-20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 09 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (Chang liver (liver cancer) cell)
Specification Chang liver (liver cancer) cell
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (egg extract)
Loading amount 10 µg
Specification egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 14 2011

Thank you for your enquiry. This product is supplied as total IgG fraction. Therefore, the antibody solution will contain specific IgG as well as other non-specific IgG serum proteins, making it impossible to determine the exact concentration. ...

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Thank you for your enquiry. I am afraid that we do not have the concentration information for this product. This antibody is provided as 100 µl of Protein A/G purified total IgG in PBS containing 0.05% sodium azide. Therefore, this product contains...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Nov 01 2006

My apologies for the delay in replying to you. The concentration of antibody has not been determined unfortunately, and there was a mistake on our datasheet concerning the recommended dilution. We recommend for western blot a dilution of 1:500.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"