重组Anti-Dlx5抗体[EPR4488] (ab109737)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4488] to Dlx5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Dlx5抗体[EPR4488]
参阅全部 Dlx5 一抗 -
描述
兔单克隆抗体[EPR4488] to Dlx5 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: C6, RAW264.7, PC12, and HeLa whole cell lysate (ab150035). IHC-P: Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4488 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109737于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/1000.
For unpurified use at 1/50 - 1/100. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols. Antigen retreval recommended; heat up to 98°C, below boiling, and then let cool for 10-20 min. |
说明 |
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ICC/IF
1/1000. For unpurified use at 1/50 - 1/100. |
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. See IHC antigen retrieval protocols. Antigen retreval recommended; heat up to 98°C, below boiling, and then let cool for 10-20 min. |
靶标
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细胞定位
Nuclear -
数据库链接
- Entrez Gene: 1749 Human
- Entrez Gene: 13395 Mouse
- Entrez Gene: 25431 Rat
- Omim: 600028 Human
- SwissProt: P56178 Human
- SwissProt: P70396 Mouse
- SwissProt: P50575 Rat
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别名
- Distal less homeo box 5 antibody
- Distal less homeobox 5 antibody
- Dlx 5 antibody
see all
图片
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Lanes 1-2 : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/5000 dilution (purified)
Lane 3 : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/10000 dilution (purified)
Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 32 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) cells labelling Dlx5 with purified ab109737 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue labelling Dlx5 with purified ab109737 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/5000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 32 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/1000 dilution (unpurified)
Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain tissue labelling Dlx5 with unpurified ab109737 at 1/100.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Lane 1 : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/1000 dilution
Lane 2 : Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384) at 1/1000 dilution
Lane 3 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution
All lanes : GST and His tagged recombinant Human Dlx5 protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer and concentration: 5% NFDM/TBST
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab109737 被引用在 13 文献中.
- Lu Y et al. Anti-Dlx5 Retards the Progression of Osteoarthritis through Inhibiting Chondrocyte Hypertrophy and Apoptosis. Evid Based Complement Alternat Med 2022:5019920 (2022). PubMed: 35280506
- Li S et al. Metallothionein 3 Promotes Osteoblast Differentiation in C2C12 Cells via Reduction of Oxidative Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33919218
- Zhang X et al. DLX5 promotes osteosarcoma progression via activation of the NOTCH signaling pathway. Am J Cancer Res 11:3354-3374 (2021). PubMed: 34249467
- Cai C et al. Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12. Regen Ther 14:245-251 (2020). PubMed: 32455154
- Twomey-Kozak J et al. Distal-Less Homeobox 5 Is a Therapeutic Target for Attenuating Hypertrophy and Apoptosis of Mesenchymal Progenitor Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32650430