Anti-DLX3抗体(ab64953)
Key features and details
- Rabbit polyclonal to DLX3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-DLX3抗体
参阅全部 DLX3 一抗 -
描述
兔多克隆抗体to DLX3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human) from an internal region
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64953于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa).
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IHC-P |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 32 kDa). |
IHC-P
1/50 - 1/100. |
靶标
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功能
Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis. -
疾病相关
Defects in DLX3 are a cause of trichodentoosseous syndrome (TDO) [MIM:190320]. TDO is an autosomal dominant syndrome characterized by enamel hypoplasia and hypocalcification with associated strikingly curly hair.
Defects in DLX3 are the cause of amelogenesis imperfecta type 4 (AI4) [MIM:104510]; also known as amelogenesis imperfecta hypomaturation-hypoplastic type with taurodontism. AI4 is an autosomal dominant defect of enamel formation associated with enlarged pulp chambers. -
序列相似性
Belongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1747 Human
- Omim: 600525 Human
- SwissProt: O60479 Human
- Unigene: 134194 Human
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别名
- AI4 antibody
- Distal less homeo box 3 antibody
- DLX 3 antibody
see all
图片
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab64953 at 1/50 dilution. Right hand panel was also treated with immunising peptide.
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All lanes : Anti-DLX3 antibody (ab64953) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab64953 被引用在 8 文献中.
- Li J et al. Effects of DLX3 on the osteogenic differentiation of induced pluripotent stem cell-derived mesenchymal stem cells. Mol Med Rep 23:N/A (2021). PubMed: 33655330
- Yang G et al. BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts. Sci Rep 7:10775 (2017). ICC/IF ; Mouse . PubMed: 28883412
- Wu LA et al. Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis. J Cell Physiol 231:1189-98 (2016). WB ; Mouse . PubMed: 26595646
- Zhang Z et al. Transcriptional factor DLX3 promotes the gene expression of enamel matrix proteins during amelogenesis. PLoS One 10:e0121288 (2015). IHC-P . PubMed: 25815730
- Gahring LC et al. Nicotinic receptor Alpha7 expression during mouse adrenal gland development. PLoS One 9:e103861 (2014). IHC-FoFr ; Mouse . PubMed: 25093893
- Wu LA et al. Development and characterization of a mouse floxed Bmp2 osteoblast cell line that retains osteoblast genotype and phenotype. Cell Tissue Res 343:545-58 (2011). ICC/IF ; Mouse . PubMed: 21271257
- Wu LA et al. Immortalized mouse floxed Bmp2 dental papilla mesenchymal cell lines preserve odontoblastic phenotype and respond to BMP2. J Cell Physiol 225:132-9 (2010). ICC/IF ; Mouse . PubMed: 20458728
- Wu LA et al. Immortalization and characterization of mouse floxed Bmp2/4 osteoblasts. Biochem Biophys Res Commun 386:89-95 (2009). ICC/IF ; Mouse . PubMed: 19501574