Dlx2 antibody (ab54725) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human lung.
Western blot - Dlx2 antibody (ab54725)
Predicted band size : 34 kDa Dlx2 antibody (ab54725) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
Flow Cytometry - Anti-Dlx2 antibody (ab54725)
Overlay histogram showing SH-SY5Y cells stained with ab54725 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54725, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-Dlx2 antibody (ab54725)参考文献
has not yet been referenced specifically in any publications.