概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to DLST
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human DLST protein (NP_001924.2, 1 a.a. ~ 453 a.a).

  • 阳性对照
    • Human liver lysate. DLST transfected 293T cell lysate. HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab72790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml. ab72790 requires purification with Protein A prior to ICC/IF.
    WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
    • 通路
      Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
    • 序列相似性
      Belongs to the 2-oxoacid dehydrogenase family.
      Contains 1 lipoyl-binding domain.
    • 细胞定位
      Mitochondrion.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2-oxoglutarate dehydrogenase complex component E2 antibody
      • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
      • Dihydrolipoamide S succinyltransferase antibody
      • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
      • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
      • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
      • Dlst antibody
      • DLTS antibody
      • E2 antibody
      • E2K antibody
      • mitochondrial antibody
      • ODO2_HUMAN antibody
      • OGDC-E2 antibody
      see all

    图片

    • Anti-DLST antibody (ab72790) at 1/500 dilution + Human liver lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • All lanes : Anti-DLST antibody (ab72790) at 1/500 dilution

      Lane 1 : DLST transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 49 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    文献

    ab72790 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab72790.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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