Anti-DLST抗体[9F4BD5] (ab110306)

概述

  • 产品名称
    Anti-DLST抗体[9F4BD5]
    参阅全部 DLST 一抗
  • 描述
    小鼠单克隆抗体[9F4BD5] to DLST
  • 经测试应用
    适用于: WB, ICC/IF, Flow Cyt, In-Cell ELISA, IPmore details
  • 种属反应性
    与反应: Cow, Human, Pig
  • 免疫原

    Full length native protein (purified) corresponding to Pig DLST. Purified α-ketoglutarate dehydrogenase complex (KGDHC) from Porcine heart.

  • 阳性对照
    • Human HDFn and HL-60 cells
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well)
IP Use at an assay dependent concentration.

靶标

  • 功能
    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • 通路
    Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
  • 序列相似性
    Belongs to the 2-oxoacid dehydrogenase family.
    Contains 1 lipoyl-binding domain.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2-oxoglutarate dehydrogenase complex component E2 antibody
    • Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex) antibody
    • Dihydrolipoamide S succinyltransferase antibody
    • Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
    • Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial antibody
    • Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex antibody
    • Dlst antibody
    • DLTS antibody
    • E2 antibody
    • E2K antibody
    • mitochondrial antibody
    • ODO2_HUMAN antibody
    • OGDC-E2 antibody
    see all

图片

  • Immunocytochemistry image of ab110306-stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Cells were then incubated with ab110306 at 1 µg/ml for 2 h at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • HL-60 cells were stained with 1 µg/mL ab110306 (blue) or an equal amount of an isotype control antibody (red) and analyzed by Flow Cytometry.
  • ab110306 staining DLST in Human monocyte-derived macrophages by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% Goat serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 1% BSA) for 12 hours at 4°C. A Cy2®-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab110306 staining DLST in Human monocyte-derived macrophages by Flow Cytometry. Cells were prepared by scraoing in PBS and fixation by paraformaldehyde. The sample was incubated with the primary antibody (1/1000 in PBS + 1% BSA) for 60 minutes at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/100) was used as the secondary antibody.
    Gating Strategy: Dead cells excluded.

    See Abreview

  • All lanes : Anti-DLST antibody [9F4BD5] (ab110306) at 1/4000 dilution

    Lane 1 : HeLa Cell lysate
    Lane 2 : Hek293T Cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    DyLight®650-conjugated Goat anti-mouse at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa


    Exposure time : 1 second

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab110306 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HELA and HEK-T293)
Loading amount
50 µg
Specification
HELA and HEK-T293
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (human MDM)
Specification
human MDM
Preparation
Cell harvesting/tissue preparation method: SCRAPING
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
dead cells were excluded
Username

Abcam user community

Verified customer

提交于 Feb 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human MDM)
Specification
human MDM
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X
Blocking step
GOAT SERUM as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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