概述

  • 产品名称Anti-Dlp抗体
  • 描述
    小鼠多克隆抗体to Dlp
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    GIDAIEIPQKPSNERVLRYCESPSVGTCCTYNMETRMAMQSRQQLEGHTK DQISRM

    , corresponding to amino acids 91/146 of Fruit fly (Drosophila melanogaster) Dlp

  • 常规说明

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 85 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性Dlp (Dally like) protein is required for proper axon guidance and visual-system function. It acts as a gatekeeper, ensuring the sharing of Wingless among cells along the dorsoventral axis. Dlp regulates wg morphogen spatial distribution and signaling. It also has a role in Hedgehog signalling in Drosphila embryonic epidermis.
  • 细胞定位Cell Membrane
  • 数据库链接
    • 别名
      • D glypican B antibody
      • Dally like antibody
      • Dally like protein antibody
      • dlp antibody

    Anti-Dlp antibody 图像

    • All lanes : Anti-Dlp antibody (ab22009) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 85 kDa

    Anti-Dlp antibody (ab22009)参考文献

    ab22009 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (Drosophila S2 R+ cells)
    Specification Drosophila S2 R+ cells
    Permeabilization No
    Fixative Paraformaldehyde
    Username

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    提交于 Oct 25 2013

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (Drosophila S2 R+ cells)
    Specification Drosophila S2 R+ cells
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Oct 24 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"