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Our Abpromise guarantee covers the use of ab7280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
The levels of DLL4 in endogenous samples may be low. Abcam has not been successful in generating western blot data using endogenous samples, but can demonstrate the specificity using recombinant proteins and overexpressing cell lysates. Some customers have been successful using ab7280 for these samples - please see published references below.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||1/10000 - 1/50000.|
This blot was produced under denaturing, reducing conditions. After transfer, the membrane was blocked for half an hour before being incubated overnight at 4°C with rabbit polyclonal to DLL4 (ab7280; diluted 1:1000). Antibody binding was detected using peroxidase labelled anti-rabbit antibody (diluted 1:40,000) for half an hour at room temperature and visualised using ECL development solution.
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