概述

  • 产品名称
  • 描述
    兔多克隆抗体to DLL4
  • 经测试应用
    适用于: IHC-P, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    AWHAPGDDLRPEAL-C

    conjugated to KLH, corresponding to amino acids 121-134 of Human Delta4.

  • 阳性对照
    • Recombinant human DLL4 protein (ab84081)

性能

应用

Our Abpromise guarantee covers the use of ab7280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50.
WB 1/500 - 1/2500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

The levels of DLL4 in endogenous samples may be low. Abcam has not been successful in generating western blot data using endogenous samples, but can demonstrate the specificity using recombinant proteins and overexpressing cell lysates. Some customers have been successful using ab7280 for these samples - please see published references below.

ICC/IF Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

靶标

  • 功能
    Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • 组织特异性
    Expressed in vascular endothelium.
  • 序列相似性
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 结构域
    The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • 翻译后修饰
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AOS6 antibody
    • Delta 4 antibody
    • delta 4 precursor antibody
    • Delta ligand 4 antibody
    • delta ligand 4 precursor antibody
    • Delta like 4 antibody
    • Delta like 4 homolog antibody
    • Delta like 4 protein antibody
    • Delta like canonical Notch ligand 4 antibody
    • Delta like protein 4 antibody
    • Delta-like 4 (Drosophila) antibody
    • Delta-like protein 4 antibody
    • Delta4 antibody
    • DLL 4 antibody
    • Dll4 antibody
    • DLL4_HUMAN antibody
    • Drosophila Delta homolog 4 antibody
    • hdelta2 antibody
    • Homeobox protein DLL-4 antibody
    • MGC126344 antibody
    • Notch ligand delta 2 antibody
    • Notch ligand DLL4 antibody
    • Notch ligand DLL4 precursor antibody
    • XDLL-4 antibody
    see all

图片

  • All lanes : Anti-DLL4 antibody (ab7280) at 1/1000 dilution

    Lane 1 : Recombinant human DLL4 at 0.05 µg
    Lane 2 : Whole cell lysate from HEK293 over-expressing human DLL4. at 10 µg
    Lane 3 : Whole cell lysate from control HEK293 . at 10 µg

    Secondary
    Peroxidase labelled anti-rabbit antibody at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa

    This blot was produced under denaturing, reducing conditions. After transfer, the membrane was blocked for half an hour before being incubated overnight at 4°C with rabbit polyclonal to DLL4 (ab7280; diluted 1:1000).  Antibody binding was detected using peroxidase labelled anti-rabbit antibody (diluted 1:40,000) for half an hour at room temperature and visualised using ECL development solution.

  • IHC image of ab7280 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7280, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab7280 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Daley-Brown D  et al. Type II Endometrial Cancer Overexpresses NILCO: A Preliminary Evaluation. Dis Markers 2017:8248175 (2017). WB, IHC, Real Time PCR ; Human . Read more (PubMed: 28659656) »
  • Jarad M  et al. Release of endothelial cell associated VEGFR2 during TGF-ß modulated angiogenesis in vitro. BMC Cell Biol 18:10 (2017). WB . Read more (PubMed: 28114883) »

See all 43 Publications for this product

客户评价及客户问答

Thank you very much for your cooperation so far and sending me the additional information.

I have one last tip i would like to share with you:

I would like to suggest to either decrease the H2O2 percentage down to 0.3% and to incubate...

Read More
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Purified protein (brain cancer)
Specification
brain cancer
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Mar 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 11 2014

Je peux vous confirmer que 0.2% Tween a été utilisé pour les immunoblots et que le lysat de pancréas de souris a été chauffé avant dépôt sur le gel de polyacrylamide.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Lung Endothelial)
Loading amount
20 µg
Specification
Lung Endothelial
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Victoria Thompson

Verified customer

提交于 May 09 2013


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Is the result as before: a strong band at the expected molecular weight of DLL4, and another smaller band at 72 kDa?

Can you tell me if the customer has tried any other DLL4 antibodies with these samples? Also, have they considered isolating...

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Thank you for contacting us.
Both of these antibodies are shipped at 4C on blue ice packs. They are both in stock, so if you order today by 8pm within the US, you will receive them by 10am tomorrow.
I hope this information is helpful to you. ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this...

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Thank you very much for your reply and your cooperation so far.

I have found your order for these two products, and would be happy to send you a replacement. Please let me know which products you have chosen from our catalog.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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