概述

  • 产品名称Anti-DLL4抗体
    参阅全部 DLL4 一抗
  • 描述
    兔多克隆抗体to DLL4
  • 经测试应用适用于: IHC-P, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    AWHAPGDDLRPEAL-C

    conjugated to KLH, corresponding to amino acids 121-134 of Human Delta4.

  • 阳性对照
    • Recombinant human DLL4 protein (ab84081)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab7280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50.
WB 1/500 - 1/2500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

The levels of DLL4 in endogenous samples may be low. Abcam has not been successful in generating western blot data using endogenous samples, but can demonstrate the specificity using recombinant proteins and overexpressing cell lysates. Some customers have been successful using ab7280 for these samples - please see published references below.

ICC/IF Use at an assay dependent concentration.
ELISA 1/5000 - 1/25000.

靶标

  • 功能Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • 组织特异性Expressed in vascular endothelium.
  • 序列相似性Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 结构域The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • 翻译后修饰Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Delta 4 antibody
    • delta 4 precursor antibody
    • Delta ligand 4 antibody
    • delta ligand 4 precursor antibody
    • Delta like 4 antibody
    • Delta like 4 homolog antibody
    • Delta like 4 protein antibody
    • Delta like protein 4 antibody
    • Delta-like 4 (Drosophila) antibody
    • Delta-like protein 4 antibody
    • Delta4 antibody
    • DLL 4 antibody
    • Dll4 antibody
    • DLL4_HUMAN antibody
    • Drosophila Delta homolog 4 antibody
    • hdelta2 antibody
    • Homeobox protein DLL-4 antibody
    • MGC126344 antibody
    • Notch ligand delta 2 antibody
    • Notch ligand DLL4 antibody
    • Notch ligand DLL4 precursor antibody
    • XDLL-4 antibody
    see all

Anti-DLL4 antibody 图像

  • All lanes : Anti-DLL4 antibody (ab7280) at 1/1000 dilution

    Lane 1 : Recombinant human DLL4 at 0.05 µg
    Lane 2 : Whole cell lysate from HEK293 over-expressing human DLL4. at 10 µg
    Lane 3 : Whole cell lysate from control HEK293 . at 10 µg

    Secondary
    Peroxidase labelled anti-rabbit antibody at 1/40000 dilution

    Performed under reducing conditions.

    Predicted band size : 74 kDa

    This blot was produced under denaturing, reducing conditions. After transfer, the membrane was blocked for half an hour before being incubated overnight at 4°C with rabbit polyclonal to DLL4 (ab7280; diluted 1:1000).  Antibody binding was detected using peroxidase labelled anti-rabbit antibody (diluted 1:40,000) for half an hour at room temperature and visualised using ECL development solution.

  • ICC/IF image of ab7280 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab7280 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7280, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DLL4 antibody (ab7280)参考文献

This product has been referenced in:
  • Veliceasa D  et al. Therapeutic manipulation of angiogenesis with miR-27b. Vasc Cell 7:6 (2015). IHC-Fr ; Mouse . Read more (PubMed: 26161255) »
  • Paiva TF  et al. Angiogenesis-related protein expression in bevacizumab-treated metastatic colorectal cancer: NOTCH1 detrimental to overall survival. BMC Cancer 15:643 (2015). IHC-P ; Human . Read more (PubMed: 26394830) »

See all 26 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Purified protein (brain cancer)
Specification brain cancer
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

提交于 Mar 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 11 2014

Je peux vous confirmer que 0.2% Tween a été utilisé pour les immunoblots et que le lysat de pancréas de souris a été chauffé avant dépôt sur le gel de polyacrylamide.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (Lung Endothelial)
Loading amount 20 µg
Specification Lung Endothelial
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. VIctoria Thompson

Verified customer

提交于 May 09 2013


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Is the result as before: a strong band at the expected molecular weight of DLL4, and another smaller band at 72 kDa?

Can you tell me if the customer has tried any other DLL4 antibodies with these samples? Also, have they considered isolating...

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Thank you for contacting us.
Both of these antibodies are shipped at 4C on blue ice packs. They are both in stock, so if you order today by 8pm within the US, you will receive them by 10am tomorrow.
I hope this information is helpful to you. ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this...

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Thank you very much for your reply and your cooperation so far.

I have found your order for these two products, and would be happy to send you a replacement. Please let me know which products you have chosen from our catalog.

Thank you for contacting us again.

I am sorry to hear that the protocol tips did not improve the results. Please let me know when you have ordered the two antibodies. In addition, did you buy them directly from us or via our distributor?
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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"