概述

  • 产品名称
    Anti-DLL1抗体
    参阅全部 DLL1 一抗
  • 描述
    山羊多克隆抗体to DLL1
  • 宿主
    Goat
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-ATQRHLTVGEEWSQD

    , corresponding to internal sequence amino acids 148-162 of Human DLL1 (NP_005609.3)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Mouse Heart and Rat Heart lysates.

性能

应用

Our Abpromise guarantee covers the use of ab85346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).

靶标

  • 功能
    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • 组织特异性
    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • 序列相似性
    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 翻译后修饰
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Delta (Drosophila) like 1 antibody
    • Delta antibody
    • Delta like 1 (Drosophila) antibody
    • Delta like 1 antibody
    • Delta like 1 protein antibody
    • Delta like protein 1 antibody
    • Delta like protein 1 precursor antibody
    • Delta, Drosophila, homolog of 1 antibody
    • Delta-like protein 1 antibody
    • Delta1 antibody
    • DL1 antibody
    • DLL 1 antibody
    • DLL1 antibody
    • DLL1_HUMAN antibody
    • Drosophila Delta homolog 1 antibody
    • H-Delta-1 antibody
    • OTTHUMP00000017690 antibody
    see all

图片

  • Western blot - DLL1 antibody (ab85346)
    Western blot - DLL1 antibody (ab85346)
    Anti-DLL1 antibody (ab85346) at 0.3 µg/ml + Rat Heart lysate at 35 µg

    Predicted band size: 78 kDa
    Observed band size: 78 kDa

文献

This product has been referenced in:
  • Chen KY  et al. A Notch positive feedback in the intestinal stem cell niche is essential for stem cell self-renewal. Mol Syst Biol 13:927 (2017). WB . Read more (PubMed: 28455349) »
  • Braune EB  et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). Read more (PubMed: 24449764) »

See all 3 Publications for this product

发表研究结果有使用 ab85346？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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1 Review or Q&A

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Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-DLL1 antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (Dll-1 Transfected CHO)
Specification
Dll-1 Transfected CHO
Fixative
Formaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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提交于 May 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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