概述

  • 产品名称Anti-DLC1抗体
    参阅全部 DLC1 一抗
  • 描述
    山羊多克隆抗体to DLC1
  • 特异性This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig
  • 免疫原

    Synthetic peptide:

    SVAIRKRSWEEHC

    , corresponding to N terminal amino acids 2-13 of Human DLC1.

  • 阳性对照
    • Human adrenal gland tissue.

性能

应用

Our Abpromise guarantee covers the use of ab21200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization.
  • 组织特异性Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.
  • 序列相似性Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 START domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHGAP 7 antibody
    • ARHGAP7 antibody
    • Deleted in liver cancer 1 antibody
    • Deleted in liver cancer 1 protein antibody
    • DLC 1 antibody
    • Dlc-1 antibody
    • DLC1 antibody
    • DLC1 Rho GTPase activating protein antibody
    • FLJ21120 antibody
    • HP antibody
    • HP protein antibody
    • KIAA1723 antibody
    • p122 RhoGAP antibody
    • RHG07_HUMAN antibody
    • Rho GTPase activating protein 7 antibody
    • Rho GTPase-activating protein 7 antibody
    • Rho type GTPase activating protein 7 antibody
    • Rho-type GTPase-activating protein 7 antibody
    • StAR related lipid transfer (START) domain containing 12 antibody
    • StAR related lipid transfer protein 12 antibody
    • StAR-related lipid transfer protein 12 antibody
    • STARD 12 antibody
    • StARD12 antibody
    • START domain containing protein 12 antibody
    • START domain-containing protein 12 antibody
    see all

Anti-DLC1 antibody 图像

  • ab21200 (5 µg/ml) staining DLC1 in Human Adrenal Gland by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows strong reticulate staining in the cytoplasm of medular cells.

  • ab21200 staining DLC1 in Human oral cancer and adjacent normal tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at 27°C; antigen retrieval was by heat mediation with citrate buffer. Samples were incubated with primary antibody (in 1% BSA in TBS) for 18 hours at 4°C. A biotinylated rabbit polyclonal was used as the secondary antibody.

    See Abreview

Anti-DLC1 antibody (ab21200)参考文献

This product has been referenced in:
  • Tripathi SC  et al. Loss of DLC1 is an independent prognostic factor in patients with oral squamous cell carcinoma. Mod Pathol 25:14-25 (2012). IHC-P ; Human . Read more (PubMed: 22002576) »
  • Carden CP  et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer. Mol Cancer Ther 11:1609-17 (2012). Read more (PubMed: 22556379) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (oral cancer and adjacent normals)
Specification oral cancer and adjacent normals
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH=6.0
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 27°C
Username

Dr. satyendra tripathi

Verified customer

提交于 Jul 18 2012

Thank you for contacting us. I am sorry to hear that you have been experiencing problems with the ab21200. In IHC-P most of the time problem lies in antigen retrieval step. This step is very crucial for IHC-P, so we always recommend our customers im...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"