The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Use at an assay dependent concentration.
功能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
组织特异性Expressed in heart, brain, skeletal muscle and lung.
序列相似性Belongs to the dickkopf family.
结构域The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
翻译后修饰May be proteolytically processed by a furin-like protease.
Hassan MQ et al. miR-218 Directs a Wnt Signaling Circuit to Promote Differentiation of Osteoblasts and Osteomimicry of Metastatic Cancer Cells. J Biol Chem287:42084-92 (2012).
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