概述

  • 产品名称
  • 描述
    兔多克隆抗体to DKK2
  • 特异性
    DKK2 fragments of ~55 kDa and ~28 kDa can also be detected in rat kidney tissue lysate
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, surrounding amino acid 241 of Mouse DKK2

  • 阳性对照
    • Lysate from Jurkat cells, 3T3 cells and rat kidney tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    ab95274 is affinity purified.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 28 kDa.

靶标

  • 功能
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 组织特异性
    Expressed in heart, brain, skeletal muscle and lung.
  • 序列相似性
    Belongs to the dickkopf family.
  • 结构域
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 翻译后修饰
    May be proteolytically processed by a furin-like protease.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dickkopf 2 antibody
    • Dickkopf gene 2 antibody
    • Dickkopf homolog 2 antibody
    • Dickkopf related protein 2 antibody
    • Dickkopf WNT signaling pathway inhibitor 2 antibody
    • Dickkopf-2 antibody
    • Dickkopf-related protein 2 antibody
    • Dickkopf2 antibody
    • DKK 2 antibody
    • Dkk-2 antibody
    • Dkk2 antibody
    • DKK2_HUMAN antibody
    • hDkk 2 antibody
    • hDkk-2 antibody
    • hDkk2 antibody
    see all

图片

  • Lane 1 : Anti-DKK2 antibody (ab95274) at 4 µg/ml
    Lanes 2 - 4 : Anti-DKK2 antibody (ab95274) at 4 µg/ml (in 1X TBS/T with 5% nonfat milk, overnight at 4C.)

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 3T3 cell lysate
    Lane 4 : Rat kidney tissue lysate

    Secondary
    Anti-rabbit conjugated secondary antibody

    Predicted band size : 28 kDa

实验方案

文献

This product has been referenced in:

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