概述
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产品名称Anti-DKK1抗体
参阅全部 DKK1 一抗 -
描述兔多克隆抗体to DKK1
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宿主Rabbit
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经测试应用适用于: WB, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human DKK1.
(Peptide available as ab101729.)
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Saos 2; HepG2.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab93017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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组织特异性Placenta.
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序列相似性Belongs to the dickkopf family.
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结构域The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 22943 Human
- Entrez Gene: 13380 Mouse
- Entrez Gene: 100157640 Pig
- Entrez Gene: 293897 Rat
- Omim: 605189 Human
- SwissProt: O94907 Human
- SwissProt: O54908 Mouse
- Unigene: 40499 Human
see all -
别名
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
see all
图片
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Western blot - Anti-DKK1 antibody (ab93017)All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml
Lane 1 :PC3 whole cell lysate (ab3954)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 29 kDa
Observed band size: 38 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-DKK1 antibody (ab93017)All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 38 kDa (why is the actual band size different from the predicted?)
Additional bands at: 200 kDa, 21 kDa, 29 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Please note that a band of similar size is reported in the literature (PMID 10383463). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (ab93017)IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
文献
ab93017 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"