Anti-DKK1抗体(ab93017)

Abreviews (1)Q&A (2)
Customer reviews and FAQs

概述

  • 产品名称
    Anti-DKK1抗体
    参阅全部 DKK1 一抗
  • 描述
    兔多克隆抗体to DKK1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human DKK1.

    参阅Abcam的专有抗源政策

    (Peptide available as ab101729.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Saos 2; HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab93017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 组织特异性
    Placenta.
  • 序列相似性
    Belongs to the dickkopf family.
  • 结构域
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody 图像

  • Western blot - DKK1 antibody (ab93017)
    Western blot - DKK1 antibody (ab93017)
    All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : PC3 whole cell lysate (ab3954)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 29 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Western blot - DKK1 antibody (ab93017)
    Western blot - DKK1 antibody (ab93017)
    All lanes : Anti-DKK1 antibody (ab93017) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,21 kDa,29 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DKK1 antibody (ab93017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DKK1 antibody (ab93017)
    IHC image of DKK1 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-DKK1 antibody (ab93017)参考文献

ab93017 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab93017？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DKK1 antibody

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample
Mouse Tissue sections (E10.5, E15.5 embryos)
Specification
E10.5, E15.5 embryos
Permeabilization
No
Fixative
Paraformaldehyde
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Verified customer

提交于 Oct 17 2013

I would like to test this antibody in rabbit, an untested species.

I am very pleased to hear you would like to accept our offer and test ab93017 in rabbit. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrabbit and include this code in the &...

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I would like to test ab93017 for use on mouse samples.

DISCOUNT CODE: *** Expiration date: February 7th, 2011 I am very pleased to hear you would like to accept our offer and test ab93017 in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, ple...

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