概述

  • 产品名称Anti-DKK1抗体
    参阅全部 DKK1 一抗
  • 描述
    小鼠单克隆抗体to DKK1
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment with tag: QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH , corresponding to amino acids 81-181 of Human DKK1

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab61275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 组织特异性Placenta.
  • 序列相似性Belongs to the dickkopf family.
  • 结构域The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody 图像

  • Anti-DKK1 antibody (ab61275) at 1 µg/ml + HeLa cell lysate at 25 µg

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab61275 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61275, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DKK1 antibody (ab61275)参考文献

This product has been referenced in:
  • Lin CL  et al. Dickkopf-1 promotes hyperglycemia-induced accumulation of mesangial matrix and renal dysfunction. J Am Soc Nephrol 21:124-35 (2010). Read more (PubMed: 20019166) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 th...

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Yes the replacement antibody would be free.

Thanks for your email.

You do not need to return the vial of ab61275. I have found your order on our system. I just want to confirm if you would like another vial of ab61275 or a different primary antibody.

Thank you!

Thanks for your reply.

It does seem like there is a problem with this vial of ab61275. If you have purchased this antibody within the last 6 months or so I would be happy to send you a replacement of any other primary antibody.

In y...

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Thanks so much for passing along the additional protocol details.

Just one more question... Have you confirmed expression of this recombinant protein by any other means, such as by western blot detection via an epitope tag or staining of the ...

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Thank you for your enquiry. I am sorry to hear this vial of ab61275 is giving you trouble.I will be able to help you more efficiently if you don't mind providing some additional detailsregarding your protocol and sample preparation in the space below. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"