概述

  • 产品名称
  • 描述
    兔多克隆抗体to DKK1
  • 经测试应用
    适用于: IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) from the internal domain of DKK1 conjugated to KLH

  • 阳性对照
    • Human uterus, endometrium.

性能

应用

Our Abpromise guarantee covers the use of ab61034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/200.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT.

靶标

  • 功能
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • 组织特异性
    Placenta.
  • 序列相似性
    Belongs to the dickkopf family.
  • 结构域
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

图片

  • ab61034 at 10µg/ml staining formalin fixed paraffin embedded Uterus (endometrium)
  • ab61034 staining DKK1 in mouse embryonic (E16.5) craniofacial tissue by IHC-Fr (paraformaldehyde-fixed frozen sections).  Paraformaldehyde fixed issue samples were permealized in 0.1% Triton X-100, blocked with 5% serum for 15 minutes at 25 °C. The sample was incubated with primary antibody dilution 1/100 in PBS at 4°C for 24 hours. An Alexa Fluor®  conjugated Goat polyclonal to rabbit IgG (dilution 1/200)  was used as secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Bleil J  et al. Granulation Tissue Eroding the Subchondral Bone Also Promotes New Bone Formation in Ankylosing Spondylitis. Arthritis Rheumatol 68:2456-65 (2016). Read more (PubMed: 27111225) »
  • Caruso M  et al. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis. PLoS One 10:e0124213 (2015). IF . Read more (PubMed: 25910078) »

See all 7 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Ahmar Aziz

Verified customer

提交于 Jul 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min at 100C
Sample
Human Tissue sections (colon cancer)
Specification
colon cancer
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 30 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample
Mouse Tissue sections (E10.5, E15.5 embryos)
Specification
E10.5, E15.5 embryos
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (mice bone cell)
Specification
mice bone cell
Permeabilization
No
Fixative
10% neutral-buffered formalin
Username

Abcam user community

Verified customer

提交于 Jun 19 2013

Thank you for your reply. Some other options for positive controls include brain tissue (cerebellum), tonsil, ovary and testis. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results for your customer. The details provided will enable us to investigate this case and will provide us with vital ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Head of Mouse Embryos E16.5)
Specification
Head of Mouse Embryos E16.5
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gaskon Ibarretxe

Verified customer

提交于 Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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