概述

  • 产品名称Anti-DKC1抗体
    参阅全部 DKC1 一抗
  • 描述
    兔多克隆抗体to DKC1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Sheep, Pig, Chimpanzee, Baboon, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human DKC1.

    (Peptide available as ab71334.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: HCT 116 Whole Cell, HeLa Whole Cell, HepG2 Whole Cell, Rat Colon Tissue, Rat Thymus Tissue, SW480 Whole Cell

性能

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应用

Our Abpromise guarantee covers the use of ab64667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
    Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
    Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation.
  • 序列相似性Belongs to the pseudouridine synthase TruB family.
    Contains 1 PUA domain.
  • 细胞定位Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBF5 antibody
    • CBF5 homolog antibody
    • Cbf5p homolog antibody
    • DKC 1 antibody
    • DKC antibody
    • Dkc1 antibody
    • DKC1_HUMAN antibody
    • DKCX antibody
    • Dyskeratosis congenita 1 antibody
    • Dyskeratosis congenita 1 dyskerin antibody
    • Dyskerin antibody
    • H/ACA ribonucleoprotein complex subunit 4 antibody
    • NAP 57 antibody
    • NAP57 antibody
    • NOLA 4 antibody
    • NOLA4 antibody
    • Nopp140 associated protein of 57 kDa antibody
    • Nopp140-associated protein of 57 kDa antibody
    • Nucleolar protein family A member 4 antibody
    • Nucleolar protein NAP57 antibody
    • snoRNP protein DKC1 antibody
    • XAP 101 antibody
    • XAP101 antibody
    see all

Anti-DKC1 antibody 图像

  • All lanes : Anti-DKC1 antibody (ab64667) at 1 µg/ml

    Lane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Colon (Rat) Tissue Lysate
    Lane 5 : Thymus (Rat) Tissue Lysate
    Lane 6 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab64667 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64667, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293, HepG2, and MCF-7 cells at 5µg/ml.

Anti-DKC1 antibody (ab64667)参考文献

This product has been referenced in:
  • Kappei D  et al. HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment. EMBO J 32:1681-701 (2013). Read more (PubMed: 23685356) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 10 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Sep 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"