概述

  • 产品名称Anti-DIXDC1抗体
    参阅全部 DIXDC1 一抗
  • 描述
    小鼠多克隆抗体to DIXDC1
  • 特异性Antibody reactive against mammalian transfected lysate.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-472 of Human DIXDC1

  • 阳性对照
    • Transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 78 kDa).
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
    • 组织特异性Ubiquitously expressed with higher expression in cardiac and skeletal muscles.
    • 序列相似性Belongs to the DIXDC1 family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DIX domain.
    • 结构域The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.
      The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.
    • 翻译后修饰Phosphorylated on tyrosine and serine residues.
      Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.
    • 细胞定位Cytoplasm; Cell junction > focal adhesion. Associated with actin stress fiber at the filament ends and Cytoplasm > cytosol. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CCD1 antibody
      • Ccd1Aalpha1L antibody
      • Ccd1Abeta1L antibody
      • Ccd1BalphaL antibody
      • Ccd1BbetaL antibody
      • Ccd1CL antibody
      • Coiled coil DIX1 antibody
      • Coiled coil protein DIX1 antibody
      • Coiled-coil protein DIX1 antibody
      • Coiled-coil-DIX1 antibody
      • DIX domain containing 1 antibody
      • DIX domain containing protein 1 antibody
      • DIX domain-containing protein 1 antibody
      • DIXC1_HUMAN antibody
      • DIXDC1 antibody
      • Dixin antibody
      • KIAA1735 antibody
      see all

    Anti-DIXDC1 antibody 图像

    • All lanes : Anti-DIXDC1 antibody (ab67763) at 1/500 dilution

      Lane 1 : DIXDC1 transfected lysate
      Lane 2 : Non transfected lysate.

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 78 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Anti-DIXDC1 antibody (ab67763)参考文献

    ab67763 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67763.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"