概述

  • 产品名称Anti-Dishevelled / Dvl1抗体
    参阅全部 Dishevelled / Dvl1 一抗
  • 描述
    兔多克隆抗体to Dishevelled / Dvl1
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Dishevelled/ Dvl1.

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Testis; Kidney; Heart. IHC-P: FFPE human testis tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 25159144
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 75 kDa).

靶标

  • 功能Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
  • 疾病相关Robinow syndrome, autosomal dominant 2
  • 序列相似性Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • 结构域The DIX domain promotes homooligomerization.
    The DEP domain mediates interaction with the cell membrane.
  • 翻译后修饰Ubiquitinated; undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
  • 细胞定位Cell membrane. Cytoplasm, cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing.
  • 别名
    • Dishevelled 1 antibody
    • dishevelled 1 like antibody
    • Dishevelled antibody
    • Dishevelled dsh homolog 1 antibody
    • Dishevelled-1 antibody
    • Dishevelled1 antibody
    • DSH homolog 1 antibody
    • Dvl 1 antibody
    • Dvl antibody
    • Dvl1 antibody
    • DVL1_HUMAN antibody
    • DVL1L1 antibody
    • MGC54245 antibody
    • Segment polarity protein dishevelled homolog DVL 1 antibody
    • segment polarity protein dishevelled homolog DVL 1 like antibody
    • Segment polarity protein dishevelled homolog DVL-1 antibody
    • Segment polarity protein dishevelled homolog DVL1 antibody
    see all

Anti-Dishevelled / Dvl1 antibody 图像

  • IHC image of Dishevelled / Dvl1 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106844, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Dishevelled / Dvl1 antibody (ab106844) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    The predicted molecular weight of Dishevelled / Dvl1 is 75 kDa (SwissProt), however we expect to observe a banding pattern around 85 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Anti-Dishevelled / Dvl1 antibody (ab106844)参考文献

This product has been referenced in:
  • Shimizu N  et al. Hipk2 and PP1c Cooperate to Maintain Dvl Protein Levels Required for Wnt Signal Transduction. Cell Rep 8:1391-404 (2014). WB, IP ; Zebrafish . Read more (PubMed: 25159144) »

See 1 Publication for this product

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