RabMAb

Anti-Dishevelled 3抗体[EP1991Y] (ab76081)

概述

  • 产品名称
    Anti-Dishevelled 3抗体[EP1991Y]
    参阅全部 Dishevelled 3 一抗
  • 描述
    兔单克隆抗体[EP1991Y] to Dishevelled 3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Dishevelled 3 aa 600-700 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab191381)

  • 阳性对照
    • K562 cell lysate and human brain tissue
  • 常规说明

    An immunizing peptide is available for this antibody as ab191381.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.Can be blocked with Dishevelled 3 peptide (ab191381).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      May play a role in the signal transduction pathway mediated by multiple Wnt genes.
    • 序列相似性
      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • 翻译后修饰
      Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dishevelled 3 (homologous to Drosophila dsh) antibody
      • Dishevelled 3 antibody
      • Dishevelled 3, dsh homolog (Drosophila) antibody
      • Dishevelled, dsh homolog 3 (Drosophila) antibody
      • Dishevelled-3 antibody
      • DSH homolog 3 antibody
      • dvl3 antibody
      • DVL3_HUMAN antibody
      • KIAA0208 antibody
      • OTTHUMP00000210777 antibody
      • OTTHUMP00000210778 antibody
      • Segment polarity protein dishevelled homolog DVL 3 antibody
      • Segment polarity protein dishevelled homolog DVL-3 antibody
      see all

    图片

    • Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
    • Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution + K562 cell lysate at 10 µg

      Secondary
      goat anti-rabbit
      HRP labelled IgG at 1/2000 dilution

      Predicted band size: 78 kDa
      Observed band size: 95 kDa (why is the actual band size different from the predicted?)

    • ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76081, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76081, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献

    This product has been referenced in:
    • Wu Y  et al. Low expression of secreted frizzled-related protein 2 and nuclear accumulation of ß-catenin in aggressive nonfunctioning pituitary adenoma. Oncol Lett 12:199-206 (2016). WB ; Human . Read more (PubMed: 27347125) »
    • He H  et al. miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/ß-catenin signaling. Int J Mol Med 35:1587-95 (2015). WB . Read more (PubMed: 25847080) »

    See all 4 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cow Tissue sections (oviductal tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
    Permeabilization
    No
    Specification
    oviductal tissue
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 28 2016

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12 % SDS PAGE gel)
    Sample
    Human Cell lysate - whole cell (HEK 293T cells)
    Specification
    HEK 293T cells
    Treatment
    transfected with Dvl-3 plasmid
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 23 2013

    Merci d'avoir pris le temps de remplir notre questionnaire. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats avec ce nouveau lot.

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    Je suis désolée d'apprendre que un nouveau lot d'ab76081 ne vous donne pas de bons résultats.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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