Anti-Dishevelled 3抗体[EP1991Y] (ab76081)


  • 产品名称Anti-Dishevelled 3抗体[EP1991Y]
    参阅全部 Dishevelled 3 一抗
  • 描述
    兔单克隆抗体[EP1991Y] to Dishevelled 3
  • 经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminal of human Dishevelled 3.

  • 阳性对照
    • K562 cell lysate and human brain tissue
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    An immunizing peptide is available for this antibody as ab191381.




Our Abpromise guarantee covers the use of ab76081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.Can be blocked with Dishevelled 3 peptide (ab191381).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能May play a role in the signal transduction pathway mediated by multiple Wnt genes.
    • 序列相似性Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • 翻译后修饰Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dishevelled 3 (homologous to Drosophila dsh) antibody
      • Dishevelled 3 antibody
      • Dishevelled 3, dsh homolog (Drosophila) antibody
      • Dishevelled, dsh homolog 3 (Drosophila) antibody
      • Dishevelled-3 antibody
      • DSH homolog 3 antibody
      • dvl3 antibody
      • DVL3_HUMAN antibody
      • KIAA0208 antibody
      • OTTHUMP00000210777 antibody
      • OTTHUMP00000210778 antibody
      • Segment polarity protein dishevelled homolog DVL 3 antibody
      • Segment polarity protein dishevelled homolog DVL-3 antibody
      see all

    Anti-Dishevelled 3 antibody [EP1991Y] 图像

    • Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
    • Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution + K562 cell lysate at 10 µg

      goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 78 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76081, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76081, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)参考文献

    This product has been referenced in:
    • Burcklé C  et al. Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. Hum Mol Genet 20:2611-27 (2011). IHC (PFA fixed) . Read more (PubMed: 21498478) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cow Tissue sections (oviductal tissue)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
    Permeabilization No
    Specification oviductal tissue
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    提交于 Sep 28 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12 % SDS PAGE gel)
    Sample Human Cell lysate - whole cell (HEK 293T cells)
    Specification HEK 293T cells
    Treatment transfected with Dvl-3 plasmid
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    提交于 May 23 2013

    Merci d'avoir pris le temps de remplir notre questionnaire. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats avec ce nouveau lot.

    Malheureusement nous ne trouvons pas le lot GR41734 - nous pensons que ceci doit pr...

    Read More

    Je suis désolée d'apprendre que un nouveau lot d'ab76081 ne vous donne pas de bons résultats.
    Veuillez trouver ci-joint le questionnaire discuté au téléphone qui nous permet de regrouper le plus d'informations le protocole que vous avez utilisé av...

    Read More