兔多克隆抗体to Dishevelled 3
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Dishevelled 3.
(Peptide available as ab108471.)
- This antibody gave a positive signal in the following lysates:
Ovary (Human) Tissue Lysate; Heart (Human) Tissue Lysate
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 mg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
May play a role in the signal transduction pathway mediated by multiple Wnt genes.
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
Information by UniProt
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
Western blot - Dishevelled 3 antibody (ab93850)
All lanes :
Anti-Dishevelled 3 antibody (ab93850) at 1 µg/mlLane 1 :
Human ovary tissue lysate - total protein (ab30222
)Lane 2 :
Human heart tissue lysate - total protein (ab29431
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
78 kDaObserved band size:
78 kDaAdditional bands at:
50 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"