重组
RabMAb

Anti-Dishevelled 2 (phospho S143)抗体[EPNCIR145] (ab124933)

概述

  • 产品名称
    Anti-Dishevelled 2 (phospho S143)抗体[EPNCIR145]
    参阅全部 Dishevelled 2 一抗
  • 描述
    兔单克隆抗体[EPNCIR145] to Dishevelled 2 (phospho S143)
  • 特异性
    ab124933 only detects Dishevelled 2 phosphorylated at Serine 143.
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Dishevelled 2 (phospho S143).
    (Peptide available as ab173138)

  • 阳性对照
    • WB: F9 and HEK293 cell lysates.
  • 常规说明

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    EPNCIR145
  • 同种型
    IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab124933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138).

For unpurified use at 1/1000 - 1/10000.

  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能
      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • 序列相似性
      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • 结构域
      The DIX domain mediates homooligomerization.
    • 细胞定位
      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Dishevelled 2 (homologous to Drosophila dsh) antibody
      • Dishevelled dsh homolog 2 antibody
      • dishevelled segment polarity protein 2 antibody
      • Dishevelled-2 antibody
      • Dishevelled2 antibody
      • DSH homolog 2 antibody
      • DVL 2 antibody
      • Dvl2 antibody
      • DVL2_HUMAN antibody
      • Segment polarity protein dishevelled homolog DVL 2 antibody
      • Segment polarity protein dishevelled homolog DVL-2 antibody
      • Segment polarity protein dishevelled homolog DVL2 antibody
      see all

    图片

    • All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)

      Lane 1 : Untreated F9 cell lysate
      Lane 2 : Alkaline phosphatase treated F9 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 78 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)

      Lane 1 : Untreated HEK293 cell lysate
      Lane 2 : Alkaline phosphatase treated HEK293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 78 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)

      Lane 1 : F9 cell lysate untreated
      Lane 2 : F9 cell lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 78 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:

    See 1 Publication for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab124933.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册